Biotech

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Biotech
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Southern Blotting

• Through a series of steps, DNA that has been
  separated by electrophoresis is applied to a
  membrane of nylon or nitrocellulose.

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PCR

• This is the polymerase chain reaction. It is a
  technique to multiply a sample of DNA many times
  in a short period of time. It supplies the
  scientist with sufficient DNA for further
  testing.
• http//wps.prenhall.com/wps/media/objects/487/4989
  29/CDA12_2/CDA12_2a/CDA12_2a.htm

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PCR diagram
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DNA fingerprinting (RFLP analysis)

• RFLP is restriction fragment length polymorphism.
  This is the idea that each persons DNA would
  cut up into different sized fragments. Thus the
  pattern produced by electrophoresis of those
  patterns would be unique to the person.
• Often probes are used to detect specific
  fragments, so the pattern is not so crowded to
  see.

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DNA fingerprinting analysis

• http//www.biology.arizona.edu/human_Bio/problem_s
  ets/DNA_forensics_1/02Q.html
• Do problems 2-4 to practice the interpretation of
  DNA fingerprints.

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Key terms to define

• Genomic Library
• DNA sequencing
• cDNA
• Human genome project
• Primer
• Hybridization
• Stem cells