Title: BCMB
1 BCMB 8110 Control of Electron Transfer
in Biology 1- Key Concepts -Transfer
Potentials -Electron transfer cofactors 2-
Overview of the electron transport
chain -Complex I -Complex II -Complex
III -Complex IV 3- Advanced Concepts in
Protein Structure/Function -Mechanisms of
redox control -Mechanisms of proton
transfer -Mechanisms of electron transfer
2Transfer Potential and the Meaning of Life
Its all about energy!
3Free Energy and Reduction Potential
4Transfer Potential
5Electron Carriers Coenzyme Q
6Electron Carriers Iron Sulfur Clusters
7Universal, Mobile Electron Carriers Ferredoxins
8Electron Carriers Low Potential Ferredoxins
9Electron Carriers Rieske Type Iron Sulfur
Clusters
10Electron Carriers Cytochromes
11Electron Carriers Cytochromes
12Electron Carriers Blue Copper Proteins
13Electron Carriers Flavoprotein
14Electron Storage in Flavins
15Complex III
16Cytochrome bc1 Complex (Cont.)
17Cytochrome bc1 Complex (Cont.)
18Cytochrome bc1 Complex (Cont.)
19Controlling Reduction Potentials,
ElectronTransfer And Proton Transfer
Rule 1, In most cases, especially for complex
metallocenters, proton and electron transfer are
coupled.
20Redox Control Rubredoxin
21Redox Control Rubredoxin
22Redox Control Rubredoxin
23Redox Control Rubredoxin
24Redox Control Rubredoxin
25Redox Control Rubredoxin
26Redox Control Rubredoxin
27Redox Control Nitrogenase
28Redox Control Nitrogenase
29Redox Control Nitrogenase
30Redox Control Nitrogenase
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32Redox Control Nitrogenase
332/1
pH-dependence for the P Redox Couple
34Model for P-Cluster Redox and Proton Transfer
Reactions
Em1
Em2
P2
P1
PN
lt -348 mV
pKa
pKa
pKa
gt 8.5
gt 8.5
lt 6.0
Em2H
Em1H
P1(H)
P2(H)
PN (H)
gt -224 mV
-310 mV
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36e-
H
8 H 8e- N2
e-
2 NH3 H2
37Cytochrome Oxidase- Proton Transfer
38Coupled Proton/Electron transfer
Cytochrome Oxidase
39Coupled Proton/Electron transfer
Cytochrome Oxidase
40Coupled Proton/Electron transfer
Cytochrome Oxidase
41Coupled Proton/Electron transfer
Cytochrome Oxidase
42Coupled Proton/Electron transfer
Cytochrome Oxidase
COX Cyc c broken beef heart mitochondria.
Capitanio et al. Biochemistry 42(16) p.4607-4612
43Coupled Proton/Electron transfer
Cytochrome Oxidase
44Electron Transfer Theory and Experiment
45Electron Transfer Theory and Experiment
46Electron Transfer Theory and Experiment
47Electron Transfer Theory and Experiment
48Electron Transfer Theory and Experiment
49Electron Transfer Theory and Experiment