Daniel Ross

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Katie Blackwell
Hema Kansara
Daniel Ross
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Blood Testing
We tested sheep blood to simulate that of an
athletes blood. This was to determine which
sample contained large traces of Erythropoietin
(EPO) to see which athlete was cheating. To
complete this practical, a haemocytometer was
used to count the red blood cells.
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Using a Haemocytometre

• Pipette 10ul of saline blood mixture into each
  of the two chambers.
• In each of the chambers you will find a grid like
  the one above.

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Method of Using a Haemocytometer

• Count the total number of cells in the four
  corner squares and the middle square and take the
  average.
• Continue the above method for each blood sample

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What else is a Haemocytometer used for?

• Cancer Research study of human cells
• Growth of cells
• Yeast cells brewing of beer
• Tripan Blue can also be used instead of a
  Haemocytometer. This is a blue dye which shows up
  the viable cells.

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Erythropoietin
What is EPO?

• Can be used to increase the amount of red blood
  cells in athletes

• a glycoprotein hormone produced primarily by
  cells of the kidney, is responsible for the
  regulation of red blood cell production.

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Erythropoietin

• training at high altitudes can achieve the same
  effects as injecting EPO into the blood system

• It is a natural drug which is stimulated by
  oxygen

• it is a banned substance in the world of sports

• it is difficult to detect as it is already
  present in the body

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For More Info

• www.glucocontrol.org/eng/lk_3776.php
• http//path.upmc.edu/consult/rla/july1995.html