Fingerprints: Nuclear DNA standard.

1
Fingerprints Nuclear DNA standard.

• FBI CODIS (Combined DNA Index System) standard
  for nuclear DNA utilizes 13 highly-variable
  tetramer STR sites.

TPOX
D3S1358
TH01
D8S1179
D5S818
VWA
Multiplexed groupings Blue, Green II,
Yellow, Cofiler
FGA
D7S820
CSF1PO
AMEL
D13S317
AMEL
D16S539
D21S11
D18S51
2
Multiplex STR Analysis
AmpFlSTR Profiler Plus
Color Separation
Size Separation
Select PCR primer sites at a distance around site
to separate the products in each dye grouping
3
Multiplex STR Analysis
Two different individuals
4
Measure against Reference Ladder
5
STR Allele Frequencies
TH01 Marker
Proc. Int. Sym. Hum. ID (Promega) 1997, p. 34