maltose

1
Maltose Binding to MBP Induces a Conformational
Change
maltose
open unliganded
closed liganded
2
Quick Quiz
MBP binds maltose with Kd 1 mM and glucose with
a Kd 10 mM. In a solution containing 1 nM MBP
and 1 mM of both sugars, which of the following
are true

• More MBP will be bound to glucose than maltose
• 50 of the maltose will be bound to MBP
• There will be less than 0.5 nM of MBP-glucose
  complex in solution

3
Structure of Myoglobin
John Kendrew 1958
4
Oxygen Binding and Heme Coordination in Myoglobin
5
Structure of Hemoglobin
Max Perutz
6
Oxygen Binding to Myoglobin and Hemoglobin
Yoxygen
7
Hill Plot for Myoglobin and Hemoglobin
8
Two States of Hemoglobin
9
Two States of Hemoglobin
10
Induced-Fit Ligand Binding Model