Effects of per on longevity and fecundity in Drosophila

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Effects of per on longevity and fecundity in
Drosophila

• James Lester
• Dr. Jadwiga Giebultowicz

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Dr. Giebultowiczs research

• Circadian Rhythms
• Clock Genes
• Tim
• Per
• Effects of expression

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Increased longevity
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Increased fecundity
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Copulating Drosophila
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My research

• Cross-breeding
• UAS Gal4 x UAS per (over expressed)
• UAS per x White
• UAS Gal4 x White
• White x White
• Wper01 x Wper01 (no expression)

• Verify UAS Gal4 x UAS per results
• Eliminate cross breeding as variable
• Determine how per increases longevity and
  fecundity

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Possible Longevity Factors

• Diet How much food is eaten?
• How much is stored?
• Ovaries Do the ovaries develop at different
  rates?
• Stress resistance
• -Heat
• -Oxidative stress
• -Metabolic stress

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Preliminary Metabolic Stress Results
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Results

• Aging of cross-bred and over expressed is
  ongoing
• Metabolic and oxidative tests are about to occur
• Aging of virgins
• The fun has just begun!!!