Cell clone DNA is equivalent to founder cell DNA

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Cell clone DNA is equivalent to founder cell DNA
The main peak of the cell clone is (with high
probability) the peak of the founder cell
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Human Cell Lineage Project

• Who cares?
• Many biologists.
• Is it possible?
• Theoretically, yes.
• Is it feasible?
• Only if process is fully automated

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Hypothetical Cell Lineage Inference Machine
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Human Cell Lineage Project

• Who cares? Many biologists.
• Is it possible? Theoretically, yes.
• Is it feasible? Only if fully automated.
• What next?
• Look at real trees, where morphology closely
  relates to lineage

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Robinia pseudoacacia experiment
One MS locus
25 samples with normal allele
3 samples with mutated allele on the same small
branch
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MMR deficiency and MS

• We need more MS mutations
• In mismatch-repair deficient organisms MS mutate
  much faster
• Analyze the impact of MMR deficiency

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Simulation of reconstructing lineage in MMR
deficient organism
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Simulation of reconstructing lineage in MMR
deficient organism
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MMR-deficient Arabidopsis experiment
23 samples 22 MS loci