Microbiology Unknown Lab BIO 732

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Microbiology Unknown LabBIO 732
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TSI agarTriple Sugar Iron Agar
A B C D E
(a) Red/red (b) control (c) Red/yellow (d)
Yellow/yellow (e) Red/yellow with H2S
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Penol Red broths
A B C D E
A (acid with gas)..B (acid)C (uninoculated
control)D (no recation) E (alkaline reaction)
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O-F tests
Control E. coli
Pseudomonas Alcaligenes
Fermenter Oxidizer
Nonsaccharolytic
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Motility Test
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Decarboxylation
uninoculated
Positive Negative
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Gelatin Hydrolysis
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Simmons Citrate
A B C
A PositiveEnterobacter B NegativeE. coli C
Control-uninoculated
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Phenylalanine Deaminase Test
neg pos
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Urea agar
pos uninoculated neg
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Methyl Red Test
A B
A Positive (E. coli) B Negative (Enterobacter
aerogenes)
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Voges Proskauer Test
A B
A Negative (E. coli) B Positive (Enterobacter
aerogenes)
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Indole Test
Negative Positive
-
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Catalase Test
-
Positive Negative
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GRAM STAIN
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Urea Hydrolysis
Positive uninoculated
Negative