NIBSC Multiplex Proficiency Panel - A multicentre study

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NIBSC Multiplex Proficiency Panel - A multicentre
study

• Clare Morris and David Padley
• Divisions of Retrovirology and Virology

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Panel History

• Panels produced for the UK National Blood
  Transfusion Authority.
• UK requirement to screen for HCV RNA in blood
  donations since 1999.
• Initially, annual HCV proficiency panels produced
  to monitor assay and operator performance across
  UK sites.
• In 2005 HIV-1 screening introduced in the one NBS
  site.
• 1st HIV-1 RNA Proficiency panel produced in 2006.
• Panels have consisted of dilutions of a single
  marker, up to 15 members/panel.

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New multiplex approach

• Panel consisted of 15 coded samples.
• Contained combinations of HIV, HCV, HBV.
• One plasma negative was included.
• 7 labs took part, 2 labs reported data using two
  systems.
• Due to potential for confirmatory testing each
  lab was sent 4 panels.
• Requested to test panel through routine
  algorithm.
• 5 labs used commercial assays, 2 used in house
  assays

Biomerieux Amplicor Procliex Ultrio In house
1 1 3 2 2
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Panel Details
Sample number Sample Concentration
1 HCV 100IU
2 HCV 50IU
3 HCV 10IU
4 HIV-1 200IU
5 HIV-1 100IU
6 HIV-1 50IU
7 Negative plasma 0
8 HIV-1, HCV, HBV 200IU, 100IU, 50IU
9 HIV-1, HCV, HBV 100IU, 50IU, 20IU
10 HCV HIV 50IU 200IU
11 HCV HIV 20IU 100IU
12 HCV HBV 20IU 100IU
13 HCV HBV 50IU 50IU
14 HIV HBV 200IU 100IU
15 HIV HBV 100IU 50IU
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HIV-1 Results
Sample 1 HCV 2 HCV 3 HCV 4 HIV 5 HIV 6 HIV 7 NEG 8 HIV HCVHBV 9 HIV HCVHBV 10 HCV HIV 11 HCV HIV 12 HCV HBV 13 HCV HBV 14 HIV HBV 15 HIV HBV
LAB 1 HCV 2 HCV 3 HCV 4 HIV 5 HIV 6 HIV 7 NEG 8 HIV HCVHBV 9 HIV HCVHBV 10 HCV HIV 11 HCV HIV 12 HCV HBV 13 HCV HBV 14 HIV HBV 15 HIV HBV
1 - - - - - -
2 Not tested for HIV Not tested for HIV Not tested for HIV Not tested for HIV Not tested for HIV Not tested for HIV Not tested for HIV Not tested for HIV Not tested for HIV Not tested for HIV Not tested for HIV Not tested for HIV Not tested for HIV Not tested for HIV Not tested for HIV
3 - - - - - -
4a - - - - - -
4b - - - - - -
5 - - - - - -
6 - - - - - - -
7a Reactive Reactive Reactive Reactive Reactive Reactive - Reactive Reactive Reactive Reactive Reactive Reactive Reactive Reactive
7b Reactive Reactive Reactive Reactive Reactive Reactive - Reactive Reactive Reactive Reactive Reactive Reactive Reactive Reactive
6
HCV Results
Sample 1 HCV 2 HCV 3 HCV 4 HIV 5 HIV 6 HIV 7 NEG 8 HIV HCVHBV 9 HIV HCVHBV 10 HCV HIV 11 HCV HIV 12 HCV HBV 13 HCV HBV 14 HIV HBV 15 HIV HBV
LAB 1 HCV 2 HCV 3 HCV 4 HIV 5 HIV 6 HIV 7 NEG 8 HIV HCVHBV 9 HIV HCVHBV 10 HCV HIV 11 HCV HIV 12 HCV HBV 13 HCV HBV 14 HIV HBV 15 HIV HBV
1 -
2 - -
3 -
4a -
4b -
5 -
6 - - -
7a Reactive Reactive Reactive Reactive Reactive Reactive - Reactive Reactive Reactive Reactive Reactive Reactive Reactive Reactive
7b Reactive Reactive Reactive Reactive Reactive Reactive - Reactive Reactive Reactive Reactive Reactive Reactive Reactive Reactive
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HBV results
Sample 1 HCV 2 HCV 3 HCV 4 HIV 5 HIV 6 HIV 7 NEG 8 HIV HCVHBV 9 HIV HCVHBV 10 HCV HIV 11 HCV HIV 12 HCV HBV 13 HCV HBV 14 HIV HBV 15 HIV HBV
LAB 1 HCV 2 HCV 3 HCV 4 HIV 5 HIV 6 HIV 7 NEG 8 HIV HCVHBV 9 HIV HCVHBV 10 HCV HIV 11 HCV HIV 12 HCV HBV 13 HCV HBV 14 HIV HBV 15 HIV HBV
1
2 Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV
3 Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV
4a Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV Not tested for HBV
4b
5
6
7a Reactive Reactive Reactive Reactive Reactive Reactive - Reactive Reactive Reactive Reactive Reactive Reactive Reactive Reactive
7b Reactive Reactive Reactive Reactive Reactive Reactive - Reactive Reactive Reactive Reactive Reactive Reactive Reactive Reactive
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Conclusions

• HIV One lab didnt detect 100IU in triplex
  sample. All other labs detected HIV at all
  concentrations.
• HCV Lab 2 failed to detect 20 IU sample. Lab 6
  failed to detect 10 IU sample 100 IU in a
  triplex sample.
• HBV 3 data sets available. All HBV samples
  detected.
• 50 proficiency panels remaining, if you would
  like to receive one please E mail me at
• Clare.Morris_at_nibsc.hpa.org.uk