Adaptamers

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Adaptamers
Thrombin-binding domain
Streptavidin-binding domain
Linking region
Thrombin
Streptavidin
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Why develop adaptamers?

• To offer a new way to target substrates to cells.
• To create a tool for studying cell-cell
  interactions.
• To provide a foundation for engineered catalysts.

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Adaptamer testing
streptavidin
adaptamer
Adaptamer A15
thrombin
anti-thrombin antibody
Control no thrombin
Bead surface
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Adaptamer quenching
Displacement strand

Results
No displacement strand added
Displacement strand added
Background fluorescence

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Streptavidin on the cell surface

• Streptavidin
• Binds strongly to biotin molecule
• Used to bind biotinylated nucleic acids or
  peptides

Big idea Streptavidin protein expressed on the
cell surface can be used to target any
biotinylated DNA/protein to the cell surface.
McDevitt, 1999
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Lpp-OmpA surface display
J. Francisco, C. Earhart, G. Georgiou, 1992
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BioBricks assemblyof protein domains
I. Phillips P. Silver 2006
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Expression of construct