Hypercarb: A New Packing Material for HPLC

1
Hypercarb A New Packing Material for HPLC

• Method Development

Toni Yorks, Irene DeGraff, Paul
Ross Thermo-Hypersil Keystone
2
Structure of Hypercarb

• Flat graphite sheets twisted without order
• Hexagonally oriented C (sp2 hybridization)

www.thermohypersil.com
3
Hypercarb vs. Silica

• No active sites (homogeneous surface)

www.thermohypersil.com
4
Goals

• Compare BetaBasic 18 with Hypercarb
• Write Hypercarb user bulletin

5
Procedure
6
Generic Gradient
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BetaBasic 18
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1 Uracil 2 Benzene 3 Toluene 4
1,2-dimethyl benzene 5 Ethyl benzene 6
1,2,3-trimethyl benzene 7 Propylbenzene 8
Butylbenzene 9 Pentamethyl benzene
Column BetaBasic 18 Length 150mm 10uL
sample T22C 1.00mL/min 254nm 230 nm Generic
Gradient
10 Pentylbenzene 11 Hexamethyl benzene 12
Hexyl benzene 13 Heptylbenzene
9
1
13
12
10
5
6
7
8
2
6
3
4
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BetaBasic 18 Results
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Hypercarb
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Column Hypercarb Length 50mm 10uL
sample T22C 1.00mL/min 254nm 230 nm Generic
Gradient
1
13
9
5
4 1,2-dimethyl benzene 5 Ethyl benzene 6
1,2,3-trimethyl benzene 7 Propylbenzene 8
Butylbenzene 9 Pentamethyl benzene 10
Pentylbenzene 11 Hexamethyl benzene 12 Hexyl
benzene 13 Heptylbenzene
12
7
10
2
8
6
4
3
1 Uracil 2 Benzene 3 Toluene
10
5
10
Hypercarb Results
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The Point

• Hypercarb distinguishes methyl and alkyl addition

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Procedure
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Atenolol
Response (mV)
2
4
6
8
Time (minutes)
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Acebutolol
Response (mV)
Time (minutes)
15
Alprenolol
Response (mV)
10
4
12
14
6
8
2
Time (minutes)
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Procedure
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BetaBasic 18 Di-substituted carboxylic acids
Column BetaBasic 18 Length 150mm 10uL
sample T22C 1.00mL/min 220 nm Generic Gradient
1 terephthalic acid 2 phthalic acid 3
isophthalic acid
3
2
1
18
BetaBasic 18 Tri-substituted carboxylic acids
Column BetaBasic 18 Length 150mm 10uL
sample T22C 1.00mL/min 220 nm Generic Gradient
1 hemimellic acid 2 trimellic acid 3 -
trimesic acid
2
3
1
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(No Transcript)
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Hypercarb Di-substituted carboxylic acids
Column Hypercarb Length 50mm 10uL
sample T22C 1.00mL/min 220 nm Generic Gradient
1 terephthalic acid 2 phthalic acid 3
isophthalic acid
2
3
1
21
Hypercarb Tri-substituted carboxylic acids
Column Hypercarb Length 50mm 10uL
sample T22C 1.00mL/min 220 nm Generic Gradient
1 hemimellic acid 2 trimellic acid 3 -
trimesic acid
2
3
1
22
(No Transcript)
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Complications/Conclusions

• Correlation between logk and logP to be used for
  user bulletin

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Future Work

• Complete basic work
• Compile data for user bulletin
• Repeat basic/acidic procedure, switch conditions