Module 4 Stream Ecology Laboratory

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Module 4 Stream Ecology Laboratory

• Nutrients-DRAFT 5-16-2003

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Measuring Nutrients in Streams

• Phosphorus
• Ortho-Phosphorus (o-P)
• Total Phosphorus (TP)
• Nitrogen
• Total nitrogen (TN)
• Dissolved inorganic nitrogen (DIN)

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Nitrogen Cycle
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Nutrients- orthoPhosphate

• Ascorbic acid method

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Perkin Elmer Lambda 3BUV/VIS spectrophotometer
Spec 20
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Standards are made from a concentrated stock
solution and diluted.
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Reagents are added to the sample, mixed then the
color is allowed to develop for 20 minutes.
vortexer
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• After the blue color develops absorbance is read
  at 880 nm.

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Ammonium-N

• Manual Salicylate Method