Re-annotation of a nitrate reductase gene cluster

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• Re-annotation of a nitrate reductase gene cluster
• Qiang He, Cristina Iftode, Toby Mapes, and
  Zhaohui Xu

Desulfovibrio vulgaris is a sulfate reducing
bacterium. Its genome has a complete nitrate
reductase according to the current annotation.
However, nitrate can neither be used as a
terminal electron acceptor nor as nitrogen
source. Even though the organism has a confirmed
nitrite reductase no biochemical evidence has
been found to support the presence of the
annotated nitrate reductase.
E.coli nitrate reductase A

• Future directions
• Mutagenesis
• Can be expanded to proteins with putative
  functions

Phylogeny of dvu1288 and dvu1289