Reductive Cleavage of Sadenosylmethionine in Radical SAM Enzymes

1
Reductive Cleavage of S-adenosylmethionine in
Radical SAM Enzymes

• The goal of the research conducted is to address
  the mechanism of cleavage of S-adenosylmethionine
  in the Radical SAM family of enzymes, with a
  particular focus on understanding the energetics
  of the reaction.
• Redox potentials of the requisite 4Fe4S
  cluster are determined in the absence of SAM and
  SAM analogs, while XAS spectroscopy is used to
  assess whether methionine coordination to the
  unique iron site of the cluster is required for
  clevage.