Parvovirus B19 Genotype 2 Plasma, Sourced from the US

1
Parvovirus B19 Genotype 2 Plasma, Sourced from
the US

• Dr Sally A. Baylis, Division of Virology
• NIBSC

2
Identification of Parvovirus Genotype 2 Plasma

• Plasma obtained at NIBSC in 2000, as a diluent
  for validation of HCV RNA assays
• Tested by conventional PCR using 3 sets of
  primers of B19V (2002)
• Retested in error in 2004, by a consensus
  real-time PCR for parvovirus B19 unexpectedly
  high titre
• DNA sequence analysis confirmed that presence of
  gt2 B19V (Accession EF216869)
• 98.29 nt identity vs IM-81 (4904 bp)
• 97.83 nt identity vs A6 (4846 bp)
• This plasma was sourced in the US it is not a
  single unit

3
Genotype 2 Titre

• The plasma has a titre of 6.2 log10 IU/ml
• Agreement between consensus in-house assay
  Artus RealArtTM Parvo B19 LC kit)
• No amplification plot in Roche assay
• 330 mls of the plasma are available
• Plasma pools associated with this sample has a
  B19V loads between 7.6-8.5 log10 IU/ml
• Products associated with the pools contain a
  mixture of gt1 gt2 B19V
• No B19V infectivity can be found in the gt2
  plasma