Multiparametric flow cytometry of

1

• Multiparametric flow cytometry of
• normal human bone marrow
• analysis and display strategies

GEIL-GTLLF 2008
Part one Leukocyte subsets A colour code is
applied trhoughout this atlas Granulocytes in
red Monocytes in green Lymphocytes in purple All
other cells, in a region of maturation defined by
the exclusion of mature cell types and thus
dubbed  bermudes  in cyan
I.1
2
Combinations
Tube 1 CD11b/CD14/CD16/CD45 Tube 7
HLADR/CD117/CD34/CD45 CD11b/CD14 p.
I-5 HLADR/CD117 p. I.29 CD11b/CD16 p.
I-6 DR/CD34 p. I.30 CD14/CD16 p. I-7
CD117/CD34 p. I.31 Tube 2 CD11b/CD117/CD34/CD4
5 Tube 8 CD34/CD56/CD19/CD45 CD11b/CD117 p.
I-9 CD34/CD56 p. I.33 CD11b/CD34 p.
I-10 CD34/CD19 p. I.34 CD117/CD34 p.
I-11 CD56/CD19 p. I.35 Tube 3
CD36/CD71/CD11c/CD45 Tube 9
CD64/CD24/CD10/CD45 CD36/CD71 p.
I-13 CD64/CD24 p. I.37 CD36/CD11c p.
I-14 CD64/CD10 p. I.38 CD71/CD11c p.
I-15 CD24/CD10 p. I.39 Tube 4
CD34/CD13/CD33/CD45 Tube 10 CD7/CD117/CD34/CD45
CD34/CD13 p. I-17 CD7/CD117 p.
I.41 CD34/CD33 p. I.18 CD7/CD34 p.
I.42 CD13/CD33 p. I.19 CD117/CD34 p. I.43 Tube
5 CD38/HLADR/CD34/CD45 Tube 11
CD22/CD10/CD20/CD45 CD38/HLADR p.
I.21 CD22/CD10 p. I.45 CD38/CD34 p.
I.22 CD22/CD20 p. I.46 HLADR/CD34 p.
I.23 CD10/CD20 p. I.47 Tube 6
CD38/CD10/CD19/CD45 Tube 12 CD4/CD2/CD56/CD45 C
D38/CD10 p. I.25 CD4/CD2 p.
I.49 CD38/CD19 p. I.26 CD4/CD56 p.
I.50 CD10/CD19 p. I.27 CD2/CD56 p. I.51
I.2
3
Leukocyte subsets display
FLy
FLx
I.3
4
CD11b/CD14/CD16/CD45 TUBE 1
I.4
5
(No Transcript)
6
(No Transcript)
7
(No Transcript)
8
CD11b/CD117/CD34/CD45 TUBE 2
I.8
9
(No Transcript)
10
(No Transcript)
11
(No Transcript)
12
CD36/CD71/CD11c/CD45 TUBE 3
I.12
13
(No Transcript)
14
(No Transcript)
15
(No Transcript)
16
CD34/CD13/CD33/CD45 TUBE 4
I.16
17
(No Transcript)
18
(No Transcript)
19
(No Transcript)
20
CD38/HLADR/CD34/CD45 TUBE 5
I.20
21
(No Transcript)
22
(No Transcript)
23
(No Transcript)
24
CD38/CD10/CD19/CD45 TUBE 6
I.24
25
(No Transcript)
26
(No Transcript)
27
(No Transcript)
28
HLADR/CD117/CD34/CD45 TUBE 7
I.28
29
(No Transcript)
30
(No Transcript)
31
(No Transcript)
32
CD34/CD56/CD19/CD45 TUBE 8
I.32
33
(No Transcript)
34
(No Transcript)
35
(No Transcript)
36
CD64/CD24/CD10/CD45 TUBE 9
I.36
37
(No Transcript)
38
(No Transcript)
39
(No Transcript)
40
CD7/CD117/CD34/CD45 TUBE 10
I.40
41
(No Transcript)
42
(No Transcript)
43
(No Transcript)
44
CD22/CD10/CD20/CD45 TUBE 11
I.44
45
(No Transcript)
46
(No Transcript)
47
(No Transcript)
48
CD4/CD2/CD56/CD45 TUBE 12
I.48
49
(No Transcript)
50
CD4/CD56
I.50
51
(No Transcript)
52
Published on ELN websiteJuly 2008

• Contributors
• Christine Arnoulet
• Marie Christine Béné
• Françoise Durrieu
• Jean Feuillard
• Chantal Fossat
• Bernard Husson
• Hélène Jouault
• Francis Lacombe
• Marc Maynadié
• Coordination
• Francis Lacombe Marie Christine Béné