R1

1
Supplemental Figure 2
2
D14-3o
ES

1.5

Relative Expression of Insulin I
1
0.5
0
Ngn3-EGFP
R1
TL1
R1
Supplemental Figure 2. Insulin I expression in
Ngn3-EGFP, parental TL1, and R1 ES cells. ES
cells were differentiated, collected on day 14 of
the tertiary culture, and analyzed for insulin I
expression by real-time RT-PCR.Expression levels
are not different compared to R1 ES-derived day
14 tertiary cultures at Pgt0.05.