Physical insulation of pathways or functions

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Physical insulation of pathways or functions
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How to build complex synthetic circuits?

• Electronic hard wired reusability of components.
• Biology is diffusive crosstalk
• We need to find a way to insulate the
  different components circuits.

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Pathway insulation methods
Orthogonality (chemical insulation) Family of
orthogonal parts Physical insulation Scaffolding
Synthetic organelles Synthetic ecosystems
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PROJECT 2BUILDING A NEW ORGANELLEFOR SYNTHETIC
BIOLOGY
A quick recap
wild-type cell
create synthesome
use synthesome
STEP 2 Recruit proteins to carryout any synbio
process of choice (e.g. drug or biofuel factory)
STEP 1 Create spatially distinct membrane
compartment that has unique molecular identity
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An external organelle
Targetting Components of a pathway to
secretion
External organelles producing cell
External organelles producing cell
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An external organelle
External organelle
Chassis (Feeder)
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An external organelle
Attachment transmembrane proteins
Chassis (Feeder)
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External organelle
Chassis (Feeder)
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External organelle
Chassis (Feeder)
communication
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External organelle composition (1)
Function?
Receiver
factory
Chassis (Feeder)
Chassis (Feeder)
sender
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Advantage part reusability
Example multicolor Hasty oscillator
State output
Chassis (Feeder)
Chassis (Feeder)
output
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External organelle composition (2)
pathway
product
Chassis (Feeder)
Starting product
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Natural example Nanoarchaeum equitans
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Nanoarchaeum ABC transporters
Chassis (Feeder)
Communication Within eukaryotes,
ABC-transporters mainly transport molecules to
the outside of the plasma membrane or into
membrane-bound organelles such as the endoplasmic
reticulum, mitochondria, etc.
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Gap junctions
Natural examples
Bacterial conjugation