Biobricked High Efficiency Recombination System

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Biobricked High Efficiency Recombination System
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Homologous Recombination
Low Efficiency lt1/104
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Site-specific SSoligo-mediated Recombination
High Efficiency 1/10
Exo 5-3 exonuclease Beta single stranded DNA
binding protein, mediate recombination Gam
inhibits RecBCD and SbcCD, linear dsDNA degrading
nucleases
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Goal

• Construction of more efficient recombination
  system through mutagenesis

DL Court, 2006
Method Error prone PCR, oligo-mediated
mutagenesis
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Advantages

• Easy readout selection on antibiotics
• Currently no biobricks for improved homologous
  recombination system
• Potential for combinatorial synthesis of
  biobricks via HR