The British Experience

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The British Experience
Mike Ansfield Veterinary Laboratories
Agency United Kingdom
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TESTS
High Temperature Rendering Enforced in UK
April 2001
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Ruminant MB ELISA
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Ruminant Compound Feed ELISA
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• Rendering temperature
• Removal of gelatine
• Test sensitivity

• Reduced test sensitivity
• Nonspecificity

Non-specific assay
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Porcine Compound Feed ELISA
Modified Counter Immuno Electrophoresis
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• Rendering temperature
• Removal of gelatine
• Test sensitivity

• Reduced test sensitivity
• Nonspecificity

Non-specific assay

• Species specificity
• Protein extraction
• Test sensitivity

MAT
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Avian compound Feed ELISA
Microscopic Analysis
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• Rendering temperature
• Removal of gelatine
• Test sensitivity

• Reduced test sensitivity
• Nonspecificity

Non-specific assay

• Species specificity
• Protein extraction
• Test sensitivity

• Species specificity
• Lack of species capability
• No link between immunoassay results and MAT
• Invisible SRM

MAT
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TaqMan? PCR
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• Rendering temperature
• Removal of gelatine
• Test sensitivity

• Reduced test sensitivity
• Nonspecificity

Non-specific assay

• Species specificity
• Protein extraction
• Test sensitivity

• Species specificity
• Lack of species capability
• No link between immunoassay results and MAT
• Invisible SRM

MAT

• Inhibitory effect
• Improving sensitivity
• Sample size

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WHERE NEXT?

• Further investigation into heat stable proteins

• Linking NIR spectrometry to MAT to combine
  visual detection of structures with a species
  identification capability

• Fluorescent Antibody approach

• Gelatin detection techniques