Animal Welfare at Carolina

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Tony WaldropVice Chancellor for Research and
Economic DevelopmentProfessor, Cell and
Molecular Physiology
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• Tracy Heenan, D.V.M.Director, Office of Animal
  Care and UseAssistant Professor,Pathology and
  Laboratory Medicine
• John Bradfield, D.V.M.Chief Veterinarian and
  Director, DLAMProfessor, Pathology and
  Laboratory Medicine
• Lesley MarsonChair, Institutional Animal Care
  and Use CommitteeProfessor of Urological Research

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UNCs commitment to compliance and adherence with
our Animal Welfare Assurance Statement.
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Our commitment

• university administration fully supports IACUC
  and DLAM and the humane care and use of research
  animals
• change in Institutional Official

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Lower threshold for non-compliance

• increased monitoring by IACUC and DLAM of
  research experiments utilizing animals
• increased reporting of non-compliance to Office
  of Laboratory Animal Welfare (OLAW)

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Increased reporting of non-compliance.
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Reportable to OLAW

• failures to adhere to IACUC-approved protocols
• initiating animal work not reviewed and approved
  by IACUC
• deviations from approved standard operating
  procedures
• animal-related activities by unauthorized or
  unqualified individuals
• ongoing, unresolved problems (e.g. overcrowding)
• shortcomings in program of animal care and use
  that are not corrected in a timely manner
• unauthorized conditions jeopardizing health and
  well-being of animals or causing death

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Potential sanctions for non-compliance with
application or established policies.
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Potential sanctions for non-compliance with
application, IACUC, or DLAM policy

• contact department Chair and/or Dean
• invalidate data gathered without approved
  application
• inspection of at-risk labs and programs at
  least monthly to ensure compliance and
  presentation of an action plan to correct
  deficiencies
• increased frequency of application review (every
  year vs. every three years)
• suspension of animal use application
• reporting to OLAW and funding agencies
• probation

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Potential non-compliance sanctions

• continued non-compliance with mouse breeding
  policy resulting in overcrowding violations
• impose fee for labor in addition to a penalty fee

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IACUC Survey

• purpose improve animal care and reduce confusion
• P.I. ideas can improve care, increase efficiency
• go to http//research.unc.edu/iacuc
• look under News and Updates

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Tracy Heenan, D.V.M.Director, Office of Animal
Careand UseAssistant Professor,Pathology and
Laboratory Animal Medicine
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The P.I. is responsible for all aspects of an
approved animal use application.
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The P.I. must

• ensure adequate training of all personnel
  handling animals on the project

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The P.I. must

• ensure that personnel are familiar with all
  aspects of the animal application

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The P.I. must

• ensure that personnel adhere to IACUC and DLAM
  policies

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The P.I. must

• ensure that personnel who speak English as a
  second language fully understand and adhere to
  the approved animal use application and to IACUC
  and DLAM policies

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The P.I. must

• ensure that breeding colonies are monitored very
  frequently by research personnel and ensure
  compliance with established breeding policies.
  Genotyping must be done early.

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• IACUC is revising the Mouse Breeding Policy.
  Look for upcoming policy change information from
  IACUC.
• required meetings for mouse breeders once policy
  is finalized

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The P.I. must

• establish and identify a lab coordinator to
  coordinate animal-related activities and to train
  lab personnel in proper animal handling
• the lab coordinator may be the P.I.

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Lab coordinators

• must attend formal training and will be certified
  by an IACUC or DLAM representative to ensure that
  investigators and other personnel are
  appropriately qualified and experienced for
  conducting procedures on living animals.

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Lab coordinators

• after the coordinator is certified, the
  coordinator can then train other individuals in
  the lab. If the P.I. changes the lab coordinator,
  he or she must inform the IACUC, and the new
  coordinator must receive the necessary training.

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The P.I. must

• establish a 24-hour contact name and phone number
  (or beeper) that DLAM can contact at any time to
  ask animal health-related questions

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The P.I. must

• conduct and document frequent monitoring of
  animals as deemed necessary by IACUC in certain
  studies

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The PI must

• arrange meeting with DLAM veterinary personnel
  and husbandry personnel to review care of and
  anticipated clinical signs in animals
• applications identified as requiring special
  monitoring by IACUC during review of application

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The PI must

• Identify animals within DLAM facilities covered
  by exceptions to approved policy
• Exceptions are deviations from approved IACUC
  or federal policy
• Exceptions are filed by investigator and must
  be approved by the IACUC

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The P.I. must

• report to IACUC any unanticipated adverse effects
  occurring in experimental animals

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The P.I. must

• contact the IACUC Training and Compliance
  Coordinator when initiating any of the following
• studies involving surgeries
• behavioral studies involving aversive
  conditioning
• studies involving animals in pain category E
• studies with unanesthetized physical euthanasia
• failure to contact the Coordinator prior to
  initiating studies is considered non-compliance
  and may result in sanctions

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The P.I. must

• place rodent carcasses in animal coolers in
  sealed bags labeled with P.I. name and
  application identification number

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Toe Clipping

• toe clipping is disallowed solely as a means of
  identification
• in all cases use of the toe clip must be
  scientifically justified in the application and
  approved by the IACUC

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• We recommend keeping records of the number of
  animals that are euthanized.

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• provide a range of possibilities in the
  application. for example
• different acceptable methods of euthanasia or
  anesthesia
• different methods of blood withdrawal
• providing alternative methods may help reduce the
  number of amendments that need to be submitted.

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New and revised IACUC policies

• How to Report Animal Mistreatment or Animal Use
  Application Noncompliance

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Amendments to an approved animal use application.
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Amendments to an approved application

• P.I. must submit in writing to IACUC any proposed
  changes to the approved procedures involving
  animals
• any changes to procedures described in the
  originally approved application must be reviewed
  and approved by IACUC prior to implementation

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Amendments to an approved application

• IACUC will handle amendments on a case-by-case
  basis
• all IACUC members will receive major amendments
• any member may request review of the amendment by
  the full IACUC in a convened meeting
• if the amendment significantly changes the
  content of the application, the P.I. must
  re-write the application for a full committee
  review

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Satellite Facility

• a non-DLAM facility in which animals are housed
  for greater than 12 hours and are cared for by
  the principal investigator

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For investigators maintaining satellite
facilities

• use DLAM computer-generated cage cards to
  identify animals
• provide DLAM with a monthly animal census
• contact DLAM veterinary personnel to report any
  animal health concerns
• provide DLAM and IACUC with a 24-hour contact
  name and number
• provide the IACUC with a key to your facility

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John Bradfield, D.V.M. Chief Veterinarian and
Director, DLAMProfessor, Pathology and
Laboratory Medicine
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DLAM policies

• investigators are responsible for working with
  DLAM staff to inform them about anticipated
  clinical signs in animals, anticipated
  phenotypes, and potential adverse effects
  resulting from experimental procedures,
  especially animals that may experience pain and
  distress

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DLAM policies

• ensure that your research personnel utilizing
  DLAM facilities are familiar with and adhere to
  DLAM policies

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DLAM policies

• wear protective clothing when working with animals

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DLAM policies

• proper use of cage cards, attention to animal
  health cards, and overcrowding cage cards

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DLAM policies

• if investigators staff is responsible for
  feeding and watering animals, the investigator
  must maintain an up-to-date log within the animal
  room

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DLAM policies

• investigators must work closely with DLAM and
  Environment, Health and Safety to coordinate
  handling of carcasses and soiled bedding in
  projects involving hazardous agents

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Lesley MarsonChair, Institutional Animal
Careand Use CommitteeProfessor of Urologic
Research
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New and revised IACUC policies.
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New and revised IACUC policies

• Carolinas IACUC policy on Cage Population
  Densities for Breeding Mice

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New and revised IACUC policies

• Rodent Tumor Burden Policy

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New and revised IACUC policies

• Carbon Dioxide Euthanasia Chamber policy

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New and revised IACUC policies

• Proper Disposal of Dead Rodents

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New and revised IACUC policies

• Euthanasia of Adult Rodents and Rodent Pups

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New and revised IACUC policies

• Guidelines for the Euthanasia of Mouse and Rat
  Fetuses and Neonates

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New and revised IACUC policies

• Rodent Tail Cut Method
• blood collection (rats, mice)

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New and revised IACUC policies

• Acceptable Methods of Rodent Blood Withdrawal

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New and revised IACUC policies

• Reviewing Requests for Exceptions to Regulations,
  Policies, and Nationally Accepted Standards

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New and revised IACUC policies

• Certification of individual performing
  unanesthetized physical euthanasia

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What you need to do!

• Appoint a lab coordinator and inform the IACUC
• Provide the IACUC with your 24-hour, 7 day a week
  contact name and number
• Ensure that your personnel are familiar with the
  content of approved applications
• Toe Clip look for transition information
• Mouse Breeders look for details on new policy
  and attend mandatory meeting

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