MICHAEL HOOKER MICROSCOPY

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MICHAEL HOOKER MICROSCOPY

• Core Facilityopen to all UNC investigators
• Confocal fluorescence/reflection/frap/fret
• Atomic Force Microscope
• Laser Micro-Dissection
• Light Microscopy tans./fluor./DIC/phase/multimod
  e/time-lapse
• http//mhmicroscopy.med.unc.edu
• Mike Chua 843-3268

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MICROSCOPY SERVICES

• UNC Core facility
• Confocal
• Laser Capture Microscopy
• Electron Microscopy
• Light Microscopy
• http//www.med.unc.edu/microscopy/
• Bob Bagnell 966-2413

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FLOW CYTOMETRY CORE

• UNC Core Facility
• Deconvolution System (3D)
• Laser Scanning Cytometer
• FACS
• http//flowcytometry.med.unc.edu/
• Larry Arnold 966-1530

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R. SUPERFINE - T. SALMON LABS

• Scanning Electron Microscopy
• Transmitted Electron Microscopy
• Atomic Force Microscopy
• 3D Force Microscopy
• Confocal Microscopy
• Light Microscopy
• rsuper_at_physics.unc.edu
• tsalmon_at_email.unc.edu

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RUSSELL TAYLOR- VISUALIZATION LAB

• Scientific Visualization
• Distributed Virtual Worlds
• Haptic Display
• Interactive 3D Computer Graphics
• UNC NIH National Research Resources for Computer
  Integrated Systems for Microscopy and
  Manipulation--
• http//www.cs.unc.edu/Research/nano/cismm/

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UNC NEUROIMAGING LABORATORYG. Gerig, A. Belger,
J. Lieberman, J. Piven

• Cognitive Neuroscience
• ERP lab Functional brain mapping
• Functional brain mapping using fMRI
• Analysis of MRI diffusion tensor imaging (DTI)
• 3D ultrasound Quantitative image analysis
• Developmental Neuroimaging UNC-NDRC Core
• High-power computer system dedicated to
  neuroimage analysis
• Neurolab http//zeus.ia.unc.edu/
• NIRL http//www.nirl.unc.edu/
• NDRC http//www.fpg.unc.edu/ndrc/cores.cfm

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BARRY WHITSEL LAB

• Intrinsic Optical Signal Imager (cell-based, in
  vivo)
• bwhitsel_at_med.unc.edu

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BIOLOGY Kerry Bloom Ted Salmon Tony Perdue

• Spinning disk Confocals
• Laser Scanning Confocals
• Transmission Electron Microscopy
• kerry_bloom_at_unc.edu
• tsalmon_at_email.unc.edu
• tdperdue_at_email.unc.edu

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BIOMEDICAL ENGINEERING

• MRS Metabolomics Imaging (J. MacDonald)
• Cortical Intrinsic Signal Imaging
• Multiphoton fluorescence Imaging
• Transmembrane Potential Imaging
• Intracellular Calcium Imaging
• Biophotonics Computer Imaging
• macdona1_at_email.unc.edu

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CELL DEVELOPMENTAL BIOLOGY J. LeMasters J.
Costello K. Jacobson

• Confocal Single 2 Photon
• Cryo Transmitted Electron Microscopy
• Scanning Electron Microscopy
• Optical Tweezers
• Fluorescence Recovery After Photobleaching
• lemaster_at_med.unc.edu
• mjc_at_med.unc.edu
• frap_at_med.unc.edu

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CHEMISTRY

• Nuclear Magnetic Resonance
• Spectroscopy
• Total Internal Reflection Microscopy Nancy
  Thompson
• nit_at_unc.edu

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DENTAL SCHOOL

• Transmitted Electron Microscopy
• Scanning Electron Microscopy
• Wallace Ambrose
• wallace_ambrose_at_dentistry.unc.edu

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PEDIATRICS/EPAJ. CARSON

• Freeze Fracture
• Transmitted Electron Microscopy
• Scanning Electron Microscopy
• Light Microscopy
• jlcarson_at_email.unc.edu

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RADIATION ONCOLOGYEdward Chaney

• CT
• Segmentation
• Registration
• 3D 4D Display
• chaney_at_med.unc.edu

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RADIOLOGY RESEARCHWeili Lin Etta Pisano
Stephen Aylward

• 3 Tesla Magnetic Resonance Imaging (Lin)
• Micro Single-Photon Computed Tomography (Lin)
• Diffraction Enhanced Imaging(Pisano)
• Computer Aided Diagnosis and Display Lab
  (CADDLab Aylward)
• -- 2D and 3D image segmentation and registration
• --Multi-modal image resgistration
• weili_lin_at_med.unc.edu etta_at_med.unc.edu
• aylward_at_unc.edu

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COMPUTER-ASSISTED SURGERY IMAGING LAB
(CASILAB)Elizabeth Bullitt

• Vessel/Tumor
• Imaging and Analysis
• Imaged Guided
• Surgery
• bullitt_at_med.unc.edu

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GENE THERAPY CENTER

• Optical Imaging (Cryocooled, luminescence and
  fluorescence)
• Allison Hawke
• allison_hawke_at_med.unc.edu

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LINEBERGER COMPREHENSIVE CANCER CENTER

• Light Microscopy
• Fluorescence Microscopy
• Steve Oglesbee
• big_rock_at_email.unc.edu

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NEUROSCIENCES NEURODEVELOPMENTAL DISORDERS
RESEARCH CENTERS

• Eva Anton - Robert Sealock
• Confocals Single 2-Photon
• Light Microscopy
• sealock_at_med.unc.edu
• anton_at_med.unc.edu

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VASCULAR BIOLOGY CENTER Cam Patterson

• Ultrasound for small animals
• cpatters_at_med.unc.edu

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MEDICAL IMAGE DISPLAY ANALYSIS GROUP (MIDAG)

• At Comp Sci, Psychiatry, Rad Onc, Radiology,
  Surgery
• Analysis Capabilities
• Image Analysis
• Image Display
• Augmented Reality (Computer Science)
• Segmentation
• Registration
• http//midag.cs.unc.edu

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NC STATE UNIVERSITY

• BOTANY DEPARTMENT Light Microscopy, Confocal,
  Nina Allen, Box 7612, NCSU
• John MacKenzie Coordinator, EM Center
  john_mackenzie_at_ncsu.edu
• Phillip Russell Dir, Analytical Instru.
  Facility Atomic Force TEM, prussell_at_ncsu.edu
• Michael Dykstra Dir, Laboratory Advanced
  Microscopy, TEM Confocal michael_dykstra_at_ncsu.ed
  u
• VET SCHOOL X-ray, CT, ultrasound, MRI
• Ian Robertson, ian_robertson_at_ncsu.edu

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DUKE-UNC BRAIN IMAGING ANALYSIS CENTER (BIAC)

• fMRI Neuroscience acquisition and analyses
• http//www.biac.duke.edu/

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POSSIBLE APPLICATIONS(from NIH proposal T. Van
Dyke B. Tsui)

• High-resolution pinhole SPECT studies
• Can do a series of images with a single injection
  depending upon the length of the half-life of the
  radionucleotide.
• Locating tumors and bone metastases
• microPET studies
• Locating a large variety of primary tumors and
  their metastases

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POSSIBLE APPLICATIONS (from NIH proposal T.
Van Dyke B. Tsui)

• MRI and MRS studies
• Tumor growth
• Changes in tumor vascularity
• Optical Imaging studies
• Luciferase localization
• Reporter gene expression
• MicroCT studies
• Tumor growth

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FUTURE DEVELOPMENTS(from NIH proposal T. Van
Dyke B. Tsui)

• Use Animal Model systems for refining specific
  imaging technologiesi.e. technologies to measure
  blood flow and microvascular permeability within
  tumors.
• Imaging results compared to histologic analyses
  can help develop technologies to measure disease
  progression and then be used to screen
  development of disease in backgrounds with
  additional genetic alterations.