"NIH Center of Biomedical Research Excellence

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COBRE - PSF
"NIH Center of Biomedical Research Excellence in
Protein Structure and Function A
Vignette" Robert P. Hanzlik, Project Leader NIH
RR-017708 www.medchem.ku.edu/COBRE/
May 6, 2006
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IDeA Programs in Kansas
1 INBRE
4 COBRE
COBRE Center for Cancer Experimental
Therapeutics (2000) Gunda I. Georg, Ph.D.,
University of Kansas (Renewal Pending)
IDeA Networks of Biomedical Research Excellence
(INBRE) (2004) Joan Hunt, Ph.D. University of
Kansas School of Medicine
Novel Approaches for Control of Microbial
Pathogens (COBRE) (2001) Opendra Narayan, DVM,
Ph.D., KU School of Medicine
COBRE Center in Epithelial Function in Health and
Disease (2002) Daniel C. Marcus, Ph.D., KSU
School of Veterinary Medicine
COBRE Center in Protein Structure and
Function (2002) Robert P. Hanzlik, Ph.D.,
University of Kansas
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COBRE-PSF The University of Kansas Kansas
State University Wichita State University
KU School of Medicine
The University of Kansas
Kansas State University
Wichita State University
KU School of Medicine
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NIH Goals for COBRE Programs

• Strengthen biomedical faculty research
  capability
• Provide flexible support to build research
  capacity
• Enhance research infrastructure
• Encourage collaborative research and research
  grant applications
• Focus on a single research area
• Foster health-related research.

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COBRE-PSF Goals

• Faculty development and research-empowerment
• Development of new research capabilities

• Faculty and student recruitment and retention
• Promote research collaboration (both intra- and
  extramural)
• Strengthen ties between Kansas Universities.

• Basic research in protein structure-function
• Establish a Center for Structural Biology
  Research.

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Focusing the COBRE Theme

• Protein Structure and Function emphasizes
• Structure (physical methods, X-ray, NMR)
• Binding interactions (ligands, proteins, DNA)
• Catalysis (mechanistic studies, inhibition)
• PTMs / Proteomics / Mass Spectrometry
• HTS possibilities
• Potential for inter- and intra-campus
  collaborations including the COBRE Core Labs

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COBRE Center in Protein Structure and
Function (2002-2007)
23 Junior Faculty participating on 4 campuses in
Kansas
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Resources - Personnel
Administration Robert P. Hanzlik, PI Mary Lou
Michaelis, Co-I Cynthia Beall, Linda Carlyle,
HBC staff
Faculty Recruits (2004) James Bann Roberto
DeGuzman Qize Wei
Investigators (2002) Brian Blagg Heather Desaire
George Bousfield Susan Egan Bill Picking Jeff
Staudinger Anna Zolkiewska
Pilot Projects (2003) David Eichhorn Audrey
Lamb Bob Rowland Asma Zaidi
Xue-wen Chen (2004) Jianwen Fang Silvia
Mora Shiguang Liu
KU 13 KSU 4 KUMC 3 WSU 3 Total 23
Emily Scott (2004) Julian Limburg Liskin
Swint-Kruse
Irina Smirnova (2005) Qize Wei
Audrey Lamb (2005) Jennifer Laurence Roberto
DeGuzman
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COBRE Center in Protein Structure and function
COBRE External Advisory Committee (5) Kathryn
Ely, The Burnham Institute Gary Gilliland,
Centocor (formerly at NIST) Brian Matthews,
University of Oregon Carol Post, Purdue
University Steven Sprang, UT Southwestern at
Dallas
COBRE Mentors (17) Glen Andrews, Andy Borovik,
Dennis Burns, Gerry Carlson, Keith Chapes, M. M.
Chengapa, Bob Hanzlik, Mike Kanost, Paul
Kelly, Cindy Larive, Mary Lou Michaelis, Russ
Middaugh, Darryl Quarrles, Mark Richter, Teruna
Siahaan, Costas Tsatsoulis, Michal Zolkiewski
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COBRE Core C Protein Purification Group
Drs. Michail Alterman, Tatyana Duzhak, Boris
Kornilaev
Overexpression of Proteins Shaker-incubators
and 10 Liter fermentor Purification of Proteins
1-100 mg or more, by FPLC Identification of
Proteins 1D, 2D and differential gel
electrophoresis, capillary HPLC, peptide mass
fingerprinting Characterization of Proteins
MALDI-TOF and TOF-TOF, HPLC Q-TOF and FTMS, IEF,
Chromatography of all kinds, Biacore Surface
Plasmon Resonance
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COBRE Core B Protein Structure Laboratory
Dr. Weijun Huang
The PSL will perform, or assist investigators,
with any or all of the following functions

• Phase determination
• Solve the structure
• Build protein model
• Refine structure
• Presentation graphics

• Attempt crystallization
• Isolate and mount crystals
• Assess crystal quality
• Collect a complete data set locally and/or at
  synchrotron

Major facilities Crystallization lab, Rigaku
X-ray Generator, major crystallographic software,
expertise in crystallography.
More details www.medchem.ku.edu/cobre
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KU Structural Biology Center
June, 2003
Dedication (Oct.'04)
Concept (Dec.'03)
Legislators, Regents, Chancellor, Provost,
KUCR/KUEA Presidents More than 120 members of the
KU Research Community
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KU Structural Biology Center
From the Northeast
3 Mass Specs, X-ray
From the Southeast
800 MHz NMR
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COBRE-PSF Statistics (Year 03, 2004-05)
Personnel Participating 23 Junior
Investigators/Mentors/Lab Groups 67 Admin. Core /
2 Scientific Cores 8 TOTAL 75
Financial Resources (direct costs) NIH COBRE
RR017708-01, -02, -02S1, -03 ca. 8 M/5
yrs Kansas Technology Enterprise
Corporation 250k/5yrs KU Deans Chairs 114k KU
Center for Research, Inc. 364/5yrs
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COBRE-PSF Statistics (Years 01-03, 2002-05)
Productivity 33 Publications, 1 book chapter, 2
patents gt50 Presentations and Invited Lectures 6
New Grants Funded 4 R01, 1 NSF, 1 ALA 3 R01
close to payline 2 R01 awaiting first review 4
Workshops, 1 Symposium (325 participants/8
states) 3 New Faculty Recruited (KU, KSU,
WSU) Remodeled 5800 f2 Research Space 17,500 f2
Structural Biology Center (Core Labs)
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COBRE - PSF
Thank you!