2005 Anatomic Pathology Exam Microscopic Slide Review

1
2005 Anatomic Pathology ExamMicroscopic Slide
Review
Presented at the57th Annual Meeting of the
ACVP December 3, 2006 Tucson, AZ
2
2005 Microscopic Slide Exam Reviewpreparing the
microscopic exam
3
2005 Microscopic Slide Exam Reviewpreparing the
microscopic exam

• Nov/Dec- initial committee meeting _at_ annual ACVP

4
2005 Microscopic Slide Exam Reviewpreparing the
microscopic exam

• Nov/Dec- initial committee meeting _at_ annual ACVP
• Mid-Apr- contributions to section leader

5
2005 Microscopic Slide Exam Reviewpreparing the
microscopic exam

• Nov/Dec- initial committee meeting _at_ annual ACVP
• Mid-Apr- contributions to section leader
• Section leader prepares draft exam
• -matrix process/tissue/species

6
2005 Microscopic Slide Exam Reviewpreparing the
microscopic exam

• May/Jun- 2nd committee meeting,
• review of draft exam, keys prepared

7
2005 Microscopic Slide Exam Reviewpreparing the
microscopic exam

• May/Jun- 2nd committee meeting,
• review of draft exam, keys prepared
• 5. Mid-Aug- slides, exam questions completed

8
2005 Microscopic Slide Exam Reviewpreparing the
microscopic exam

• May/Jun- 2nd committee meeting,
• review of draft exam
• 5. Mid-Aug- slides, exam questions completed
• 6. Sep- exam time

9
2005 Microscopic Slide Exam Review

• 1. rat, brain, astrocytoma
• horse, artery, strongylosis
• dog, skin, ceruminous gland carcinoma
• dirofilariasis
• 4. dove, liver, amyloidosis
• sea lion, kidney, leptospirosis

10
2005 Microscopic Slide Exam Review

• ox, lung, atypical interstitial pneumonia
• cat, intestine, panleukopenia FIP
• marmoset, bone, fibrous osteodystrophy
• dog, salivary gland, infarct
• ox, spinal cord, fibrocartilaginous embolus

11
2005 Microscopic Slide Exam Review

• cat, kidney, lily toxicosis
• dog, eye, phthisis bulbi
• rhesus, lung, SIV CMV
• walleye, skin, fibrosarcoma
• ox, heart, ionophore toxicosis

12
2005 Microscopic Slide Exam Review

• dog, bone, metastatic carcinoma
• horse, placenta, aspergillosis
• dog, liver, autoimmune hemolytic anemia
• rat (panel), thoracic mass, hibernoma
• ox (TEM), oral mucosa, MCF

13
Case 7 Tissue from a cat.

• Histopathologic description (12 points)
• Morphologic diagnosis(es) (4 points)
• Cause(s) (4 points)

14
(No Transcript)
15
(No Transcript)
16
(No Transcript)
17
(No Transcript)
18
(No Transcript)
19
Case 7 Tissue from a cat.

• Histopathologic description (12 points)
• Intestine
• Mucosa
• Blunting of villi
• Collapse of lamina propria
• Dilated crypts with necrotic debris
• Muscularis, serosa, mesentery
• Perivascular accumulations of PMNs, macrophages
  and fibrin
• O/C
• Logical and clear presentation of features

20
Case 7 Tissue from a cat.

• Morphologic diagnosis(es) (4 points)
• Necrotizing enteritis
• Pyogranulomatous perivasculitis/peritonitis
• Cause(s) (4 points)
• Feline parvovirus (Feline panleukopenia virus)
• Feline infectious peritonitis virus

21
Case 9 Tissue from a dog.

• Histopathologic description (14 points)
• Morphologic diagnosis(es) (6 points)

22
(No Transcript)
23
(No Transcript)
24
(No Transcript)
25
(No Transcript)
26
Case 9 Tissue from a dog.

• Histopathologic description (14 points)
• Salivary gland (ID)
• Vasculature
• Thrombosis
• Acini
• Coagulative necrosis
• Inflammation
• Granulation tissue

27
Case 9 Tissue from a dog.

• Histopathologic description (14 points)
• Salivary gland (ID)
• Septa
• Inflammation
• Hemorrhage
• Fibrosis
• Ducts
• Inflammation
• Necrosis
• Squamous metaplasia
• O/C
• No points allotted

28
Case 9 Tissue from a dog.

• Morphologic diagnosis(es) (6 points)
• Necrotizing sialoadenitis with thrombosis
  (infarction) and squamous metaplasia

29
Case 11 Tissue from a cat.

• Histopathologic description (13 points)
• Morphologic diagnosis(es) (4 points)
• Most likely cause (3 points)

30
(No Transcript)
31
(No Transcript)
32
(No Transcript)
33
(No Transcript)
34
Case 11 Tissue from a cat.

• Histopathologic description (13 points)
• Distribution
• Tubular epithelium
• Degeneration
• Necrosis
• Regeneration

35
Case 11 Tissue from a cat.

• Histopathologic description (13 points)
• Casts
• Granular
• Protein
• Mineralization
• O/C
• Logical and clear presentation of features

36
Case 11 Tissue from a cat.

• Morphologic diagnosis(es) (4 points)
• Diffuse tubular necrosis with casts
• Most likely cause (3 points)
• Lily toxicosis
• Aminoglycoside antibiotic toxicosis

37
Case 16 Tissue from a dog.

• Histopathologic description (14 points)
• Morphologic diagnosis(es) (6 points)

38
(No Transcript)
39
(No Transcript)
40
(No Transcript)
41
(No Transcript)
42
(No Transcript)
43
(No Transcript)
44
Case 16 Tissue from a dog.

• Histopathologic description (14 points)
• Bone
• Neoplasm location, pattern, cell morphology,
  cell borders, cytoplasm, nuclear morphology,
  nucleoli, mitoses, anisokaryosis, necrosis
• Proliferation of new bone
• Resorption of trabecular bone

45
Case 16 Tissue from a dog.

• Histopathologic description (14 points)
• Muscle degeneration and atrophy
• O/C
• Logical and clear presentation of features
• Primary vs. secondary processes

46
Case 16 Tissue from a dog.

• Morphologic diagnosis(es) (6 points)
• Metastatic transitional cell carcinoma in bone
• Hyperostosis
• Muscular atrophy and degeneration

47
Case 19 Tissue from a rat.

• Microscopic description (16 points)
• Morphologic diagnosis (4 points)

48
(No Transcript)
49
(No Transcript)
50
(No Transcript)
51
(No Transcript)
52
Case 19 (panel) Tissue from a rat.

• Microscopic description (16 points)
• A. HE
• Description pattern, cell morphology, cell
  borders, cytoplasmic vacuoles (fat), nuclear
  morphology, multinucleated cells, nucleoli, no
  mitotic figures
• B. Oil-Red-O
• Positive cytoplasmic staining lipid

53
Case 19 (panel) Tissue from a rat.

• Microscopic description (16 points)
• C. Mitochondrial uncoupling protein-1
  immunohistochemistry
• Reactivity indicates presence of numerous
  mitochondria

54
Case 19 (panel) Tissue from a rat.

• Microscopic description (16 points)
• D. TEM
• Many mitochondria
• Some mitochondrial swelling
• Fat droplets
• Morphology consistent with brown fat

55
Case 19 (panel) Tissue from a rat.

• Morphologic diagnosis (4 points)
• Hibernoma

56
2005 Microscopic Slide Exam Reviewscoring
Proportion () Passed Mean Score
Range of Scores