A Quick Tour of the Genomics Frontier

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A Quick Tour of the Genomics Frontier

• Thane Kreiner, Ph.D.
• Affymetrix, Inc.

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Overview

• The Human Genome Project
• Tools to Understand Genomes
• Healthcare Applications of Genomics
• Potential Environmental Genomics

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Human Genome Project
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DNA
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The Complexity of the Genome

• The genome is complex
• 30,000 to 40,000 genes
• only 1.5 is genes
• introns and exons
• splice variants

• Next step is understanding genome
• variability
• function

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Understanding Genomes
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Tools to Understand Genomes
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Tools to Understand Genomes Arrays
Sequence Selection
Mask Creation
Array Manufacture
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Manufacturing Process Video
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Tools to Understand Genomes Wafer and Chip Format
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Whole Genome Expression Analysis

• 33,000 human genes on two chips
• 1 million features

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Whole Wafer Genome Scanning

• Organized view of patterns across genome

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Healthcare Applications Drug Discovery
Development
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Healthcare Applications Preclinical Toxicology
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Healthcare Applications Cancer Prognosis
Gene expression associated with good prognosis
60 Medulloblastoma samples
Gene expression associated with bad prognosis
Pomeroy, S.L. et al. (2002)
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The Genetic Age
Genetic Information
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Environmental ApplicationsMicroorganism
Detection
Wilson, W.J. et al. LLNL
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Environmental ApplicationsEcosystem Health
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Environmental Applications of Genomics

• Pathogen detection and classifications
• Water, air, soil

• Environmental toxicology
• Human
• Animal
• Ecosystem

• Ecosystem diversity

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Tools to take you as far as you vision