Project 200007900: Assess Resident Fish Stocks

1
Project 200007900 Assess Resident Fish Stocks
of the Owyhee/Bruneau Basin on the Duck Valley
Indian Reservation Mattie Allen, Shoshone
Paiute Tribes
2
Duck Valley Indian Reservation
3
(No Transcript)
4
Coastal Rainbow Trout and Redband Trout
Distributions
5
Redband Trout Distribution and Status in the
Owyhee Subbasin
6
Past Activities in the East Fork Owyhee River
Basin
Grazing Practices
Hatchery Releases

• Hatchery coastal rainbow trout stocked over a
  period of several decades

• Riparian corridors remained
• unprotected despite heavy grazing pressure

7
Goal Preserve native redband trout populations
in East Fork Owyhee River and its tributaries.
8
Objectives

• Evaluate the genetic variation within and among
  putative native redband trout populations from 14
  locations in the upper East Fork Owyhee River
  watershed
• Evaluate whether Fx progeny from events of
  introgression between hatchery rainbow trout and
  native redband trout or subsequent introgressed
  generations are present in 14 locations in the
  upper East Fork Owyhee River watershed

9
Location of Upper East Fork Owyhee River
Collection Sites
10
Genetic Analyses

• mtDNA
• Gene regions
• CytB and ND2
• Restriction enzymes
• Ava I, Dde I, Hae III
• Dpn II, Hha I, Hinf I,
• Mse I, Msp I, Rsa I,
• Taq I,

• Microsatellites
• Primers
• Omy 7
• Omy 8
• Omy 10
• Omy 34
• OMM 1020

11
Composite Mitochondrial Haplotype Frequencies for
Putative Redband Trout Populations from Upper
East Fork Owyhee River
Composite Mitochondrial Haplotypes
12
Percentage Sequence Divergence Among Observed
mtDNA Haplotypes from the Upper East Fork Owyhee
River
13
Inferred Evolutionary Relationships Among mtDNA
Haplotypes in the Upper East Fork Owyhee River
II
I
III
V
IV
VI
0
1.0
2.0
Percent Sequence Divergence
14
Composite Mitochondrial Haplotype Frequencies for
Putative Redband Trout Populations from Upper
East Fork Owyhee River
Composite Mitochondrial Haplotypes
15
Management Implications and Emerging Issues
?