IACUC

1
IACUC

• Institutional Animal Care and Use Committee
• Illinois State University

2
Regulatory Agencies

• The following agencies are responsible for
  overseeing animal research at ANY United States
  institution, public OR private (including drug
  corporations)
• USDA United States Department of Welfare
• AWA Animal Welfare Act
• OLAW Office for Laboratory Animal Welfare
• NIH and NSF
• AAALAC association for the Assessment and
  accreditation of laboratory animal care
• AALA American Association of Laboratory Animal
  Science

3
The IACUC committee is composed of

• Research Ethics and Compliance officer (non
  voting)
• Chairperson
• Veterinarian
• Scientific Animal Users (4 to 6)
• Non scientific non user
• Community member
• Committee members are appointed by President of
  University to oversee all animal care and use
• ALL animal facilities labs and farm
• Can suspend any research/animal use that is not
  in compliance

4
Role of the IACUC

• Protocol Review
• review applications for animal research
• Program Review
• review ongoing programs of research
• Facilities Review
• oversee and inspect any facility used in animal
  research
• Address animal concerns
• Continue to participate in training regarding
  maintenance and protection of animals used in
  animal research
• Address facilities needs as changes in
  regulations occur
• Ensure that all facilities and research remains
  in compliance with federal regulations
• Review and act on any concern regarding the use
  of animals in research brought to the attention
  of the IACUC
• Must meet at least twice a year generally meet
  monthly
• Inspect all animal use facilities (vivarium,
  labs, farm) at least twice yearly

5
Areas of Oversight

• General Animal use
• Who and what
• Where, how, when and how many
• Healthy Animal procedures
• Surgery procedures
• Record keeping for animals
• Drugs DEA licensing and oversight
• Euthanasia training and oversight
• Occupational health and safety
• Chemical use
• Certifications for staff researchers and
  caretakers
• Training
• General IACUC policy/procedure
• Care and handling of animals
• Housing and general care of animals

6
IACUC protocols

• Each animal use protocol must include a clear
  description of all procedures to be conducted on,
  housing,and care of the animals.
• Deal with several different issues than IRB
  protocols
• Animal species, number and source
• Pain and stress classification and justification
• Housing
• Infectious disease, chemicals, food chain issues,
  etc.
• Description of study/experimental methods
• Justification of numbers/species/alternatives to
  animal research
• Prevention/monitoring of Pain and distress
• Euthanasia

7
Pain and Stress Classifications

• Classification A No pain, distress, or use of
  pain-killing drugs. (i.e. post-mortem tissue
  harvest and routine procedures causing only
  transitory discomfort such as venipuncture,
  injections, ear tagging, use of non-inflammatory
  adjuvants)
• Classification B Pain/distress with appropriate
  analgesic/anesthesia/tranquilizers. Procedures
  involving accompanying pain or distress to the
  animals and for which the appropriate anesthetic
  (for surgery), analgesic (for inflammation or
  pain) or tranquilizing drugs are used. (YOU MUST
  COMPLETE APPENDIX B)
• Classification C Pain/distress without
  appropriate analgesic/anesthesia/tranquilizers.
  Procedures involving accompanying pain or
  distress to the animals and for which the
  appropriate anesthetic, analgesics or
  tranquilizing drugs are not used. (YOU MUST
  COMPLETE APPENDIX B)
• Federal guidelines require that alternatives are
  consider for all procedures that cause more than
  momentary pain or distress to animals

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Must be able to answer to several following
committees and issues

• _____Yes _____No Behavioral Studies
• _____Yes _____No Trauma
• _____Yes _____No Client-owned animals
  (provide a copy of the consent form the owners
  will sign)
• _____Yes _____No Animals are sent to
  slaughter or put into the human food chain
• _____Yes _____No Euthanasia and harvesting of
  tissue only
• _____Yes _____ No Chemicals (i.e.
  carcinogens, mutagens, reproductive toxins, or
  highly toxic
• substances)
• _____Yes _____No Radiation (i.e. irradiation,
  radio labeled substances, sealed radioactive
  sources, lasers, etc.)
• _____Yes _____No Infectious Agents
  (bacterial, viral, fungal, etc, human blood and
  bodily fluids are
• 
  considered potentially
  infectious)
• _____Yes _____No Use of recombinant DNA
• For any of the items below checked yes, the
  applicant must also attach the designated
  completed appendices (All protocols must include
  a completed Environmental Health Safety Risk
  Assessment, Appendix A)
• _____Yes _____No Included a completed
  Appendix A
• _____Yes _____No Animals classified in stress
  levels B or C from Section II Appendix B
• _____Yes _____No Housing of animals outside
  of the approved housing areas for more than 12
• hours Appendix C
• _____Yes _____No Survival Surgery Appendix
  D

9
Which animals must have an IACUC approval before
you can use them?

• Any vertebrate animal
• Rodents
• Any mammal!
• Fish and reptiles
• Not crustaceans
• Not insects
• Approval is granted to
• Faculty only
• For up to three years (in yearly re-approvals)
• Must reapply with entire new protocol after three
  years
• All users must be listed on protocol and be
  trained in IACUC and animal care and use
  procedures.
• Any change in procedures must be submitted in
  writing and approved by the IACUC before they can
  be implemented.
• Binding signature PI is essentially signing a
  contract stating what they will do.

10
How are hazards handled?

• Anyone working with biohazards, including tissue,
  specimens, any sharps, chemical, radiation, etc.,
  must follow Bloodborne Pathogen plan
• All personnel must wear protective clothing when
  working with biohazards
• All incidents must be reported
• bites, sticks, spills, etc.
• Reports must be maintained by each lab
• If significant injury or accident, to Dept. chair
  and Assoc Director of Research
• Each PI must provide training in safety and
  appropriate procedures
• Common sense rules!
• Wash hands
• Dont eat/drink while handling animals
• No smoking!
• Etc.

11
Who can use animals and enter animal facilities?

• Only approved personnel are allowed access
• Provides limited access to prevent contamination
  and prevention of disease
• Ensures safety of the animals
• Must have passed training!
• All animal users MUST complete their required
  CITI online training prior to using an animal
• Each animal user must also complete any necessary
  training regarding particular procedures prior to
  independently conducting that procedure.
• Each lab is responsible for maintaining training
  records
• The Research Ethics and Compliance officer
  maintains records regarding CITI training
• Access may be allowed with special permission
  from IACUC chair or Assist Director of
  Research/Research Ethics and Compliance officer
• Keys only issued by Research and Sponsored
  Programs.

12
Care of animals

• Any concern regarding an animal should be
  immediately reported to RSP, any IACUC member, or
  the consulting vet.
• Emergency numbers are located in the vivarium
• Each lab is responsible for maintaining emergency
  numbers list
• ALL research personnel are responsible for
  reporting injury, illness or other issues!
• Vet consultation required for any category B or C
  procedure BEFORE it is implemented.
• Oversite by Veterinarian is required
• Veterinarian may require monitoring, as specified
  in IACUC protocol.

13
What animals do we have at ISU?

• Farm animals
• Cows, pigs, sheep, etc. (NO horses)
• Laboratory animals
• Rats
• Hamsters
• Guinea pigs
• Rabbits
• Mice this is the only species we breed
• Reptiles and Fish

14
How are animals housed?

• As much as possible in social housing, if
  appropriate for the species
• Each has a cage card with
• Date of birth
• Date of arrival
• Species
• Principal investigator
• IACUC approval number
• Also use other permanent/semi-permanent markings
  Sharpe markers or dye
• Any deprivation conditions must be clearly
  lablelled
• E.g. notices to animal care staff Do not feed/do
  not water
• This indicates it is the PIs responsibility for
  feeding.
• Special regulations regarding diet, lighting,
  temperature and humidity are regulated by USDA,
  AWA and NIH/NSF
• We require shoe covers, lab coats and gloves to
  be worn when in the vivarium. Also, please use
  shoe disinfectant when entering vivarium. This
  keeps things clean!

15
Emergency numbers

• It is the responsibility of all animal users to
  report a sick/injured or distressed animal
• Contacts use the emergency numbers posted on the
  wall of the vivarium or your laboratory
• Your immediate supervisor
• Animal Care Technician
• IACUC Chairperson
• Veterinarian
• Research Ethics and Compliance Officer
• ISU Police
• If in doubt, report.