Answers to Gel Electrophoresis Lab

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Answers to Gel Electrophoresis Lab

1. The colors green and black do not exist on the
   gel after electrophoresis. They dont because
   the green was broken down into blue and yellow.
   The black was broken down into all colors (black
   is a mixture of all colors)
2. Yellow dye is the smallest molecule because it
   was farthest away from the wells. It was able to
   move the fastest and get farthest away

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Answers contd

• 5. Blue molecules were the biggest because they
  were closest to the wells
• 6. The pigments have negative charge because
  they move from the negative towards the positive
  end of the electrophoresis chamber.
• 7. If used DNA would have to use restriction
  enzymes to create the different sized pieces of
  DNA.

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Answers contd.

• 3 applications in which DNA fingerprint or gel
  electrophoresis may be useful
• -- ID criminals
• -- maternity/paternity
• -- evolutionary relationships
• -- ID remains of the dead
• -- Army ID

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For test

• Dont need to know the exact results (for example
  that blue dye was the biggest) need to know the
  concepts (the bigger the molecule the less
  distance it moves)
• Reread and study the procedure and background
  information for all labs

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Answers to packet page 9-10

• A, any cell would have same DNA
• B. mRNA
• C. Copy mRNA --gt ssDNA --gt dsDNA
• D. DNA polymerase
• E. Cut DNA (gene) and plasmid with same
  restriction enzyme

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• F, Cut plasmid and gene with R.E.
• G. HIND III- palindrome exists at each end of
  gene and on plasmid
• H. HIND III - must use same enzyme to cut
  plasmid as was used to cut gene
• I. They are the same
• J. A ------- TTCGA
• AGCTT A

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• K. The sticky ends are complementary
• L. Make sure base pairs are complementary
• M. Insert plasmid into bacterium (E. Coli)
• N. The gene would either not have produced any
  protein or the incorrect protein

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Are you my mother? Lab

• Gel electrophoresis - used to determine
  variations in DNA sequences
• The mom and and dads DNA fingerprint wont match
  the babys exactly. This is because 1/2 the DNA
  came from mother, 1/2 came from father

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Review

• 1. Be able to go from mRNA to DNA and DNA to
  mRNA
• 2. What is ligase? Reverse transcriptase? DNA
  polymerase?
• Whats reverse transcription?
• What is a plasmid? Where can it be found? In
  which of the labs was it needed?
• What 2 things determine how a molecule (dye or
  DNA) will move in gel electrophoresis?