Mus musculus : Transgenic Tools to Study Mice

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Mus musculus Transgenic Tools to Study Mice

• By Josh Streets
• Sam Elliott
• Bonnie Hunt

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Why Mice Are Used

• Resembles human genome in size and content
• Mouse life cycle is representative of the
  mammalian life cycle
• Has many genetically defined lines
• Many progeny

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Transgenic Techniques

• Add-on
• Inserting foreign DNA into the germ line
• No limits to the type of DNA incorporated
• Correlate mutant phenotypes with specific
  transcription units

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Transgenic Techniques

• Knockout
• Replaces the wt allele
• Produces an allele with an altered function

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The Uses of Transgenic Techniques

• Gene Function
• Express the natural transgene product in an
  unnatural manner
• Use the abnormal level, time, and place of
  expression to find the normal function of the
  wild type gene
• Preferred use in-vivo due to natural cell and
  gene interactions

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The Uses of Transgenic Techniques

• Characterize Regulatory Regions
• Helps determine the location sequence of gene
  regions
• Genomic clone of the gene of interest is flanked
  by sequences that contain the regulatory region
• Reporter gene is used to locate the gene of
  interest

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The Uses of Transgenic Techniques

• Link Mutant Phenotypes to Specific Transcription
  Units
• Establish whether a cloned gene corresponds to a
  locus previously defined mutant phenotype
• If the transgene can correct the mutant phenotype
  it proves that the presence of the mutant
  phenotype is equivalent to the absence of the
  wild type

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