QIMR Core Facilities and Technology Platforms

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QIMR Core Facilities and Technology Platforms

• Juan Cooper
• Scientific Manager
• Contact juan.cooper_at_qimr.edu.au

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QIMR Core Facilities and Technology Platforms

• Core Scientific Services
• Animal Facility
• Histotechnology Facility
• ACRF Cellular Imaging Centre
• Gene technology platforms
• Protein technology platforms
• Protein Discovery Centre
• Q-Gen Facility

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Scientific Services

• Responsible for
• Core Facilities
• Specialised Equipment

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DNA/Peptide Technology

• Contact Macky Edmundson
• macky.edmundson_at_qimr.edu.au
• Automated DNA sequencing
• Peptide chemistry
• Immunogen preparation
• Liquid chromatography (HPLC)

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Animal Facility

• Contact Sue Cassidy
• Suzanne.Cassidy_at_qimr.edu.au
• Experimental Holding
• Transgenic Breeding
• AQIS Quarantine
• Micro-isolator containment
• Animal Imaging
• Animal Irradiation

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Biophotonic Imaging

• Xenogen IVIS 100
• Cellular, genetic activity,
• in vivo luciferase
• Living Image software
• Anaesthesia system

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Irradiator

• MDS Nordion GammaCell 40
• Mouse irradiation
• Cell irradiation

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Transgenic and Knockout Mouse Facility

• Contact Dr Graham Kay
• Graham.Kay_at_qimr.edu.au
• Production of genetically modified mice
• Targeting and transgenic expression constructs
• Transgenic mouse lines specific for genome
  engineering
• Collaborative research/contract service

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Transgenic and Knockout Mouse Services

• Transgenic mice
• Knockout mice
• TS-Knockout mice
• Knockout crosses
• Blastocyst injection

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Histotechnology Facility

• Contact Clay Winterford
• Clay.Winterford_at_qimr.edu.au
• Joint QIMR/UQ SOM
• Provides training, technical services and
  consultation
• Histology
• Immunohistochemistry
• Transmission EM

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Histotechnology Facility

• Histology
• Shandon Excelsior Tissue Processor
• Shandon Varistain Gemini
• Bond maX Automated Histochemistry

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Histotechnology Facility

• EM
• Jeol 1200EXII
• Mag 50-600 000x. 
• Leica Ultracut UCT ultramicrotome
• Leica Lynx automatic tissue processor
•  

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Pathology Slide Scanner

• Aperio ScanScope T2
• Automated slide analysis
• 120 Slide capacity
• 20 X or 40 X objectives
• Specimen or tissue microarray
• Digital slide repository
• Remote viewing via internet

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Laser Capture Microscope

• P.A.L.M. LPC-Microbeam
• Isolate specific cells in
• tissue sections
• Cut out and catapult
• Ablate unwanted cells

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ACRF Cellular Imaging Centre

• Contact Grace Chojnowski
• Grace.Chojnowski_at_qimr.edu.au
• Flow Cytometry
• analysis, sorting
• Confocal Microscopy
• Digital Imaging
• fluorescence microscopy

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High-Speed Cell Sorting

• DakoCytomation MoFlo
• purchased 2003
• 12 parameters
• 3 laser beam, 9 colour
• 10,000-100,000 cells/sec
• BD FACSAria
• ordered Nov 2006
• 16 parameters
• 3 laser beam
• up to 13 colours
• Class I enclosure

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Confocal Microscopy

• Bio-Rad MRC 1024
• inverted
• Leica TCS SP2
• purchased 2002
• upgraded to two photon 2004
• Chameleon laser 720 950 nm
• 5 detectors (4 fluor, 1 trans)
• thick specimens

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Fluorescence Microscopy

• Leica DMIRB
• inverted, fluorescence
• Leica MZ FL III
• dissecting, fluorescence
• Zeiss Axioskop 2 Plus MOT
• upright stage
• high sensitivity camera

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Real-Time Time-Lapse Fluorescence Microscopy

• Olympus Cell IR
• (ordered)
• live cell
• time-lapse
• real-time fluorescence
• CO2 incubator chamber
• warming plate

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Gene Technology Platforms

• High-end technology platforms

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Real-time PCR

• 1 x AB Prism 7700
• 3 x Corbett Research RotoGene 3000
• 1 x Corbett Research RotoGene 6000

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High-Throughput Genetic Screening

• Varian Helix DHPLC
• Automated genetic screening
• Mutation detection
• SNP detection
• DNA fragment sizing
• PCR product quality control

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Microarray Facility

• Contact Dr Rick Woods
• Rick.Woods_at_qimr.edu.au
• Array preparation
• Genetic Microsystems GMS418
• Array Reading
• Genetic Microsystems GMS418
• GeneSprint software

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Agilent Microarray Scanner

• Agilent G2565BA
• 48 slide (25 x 75 mm)
• 2 colour data acquisition
• 5 or 10 micron
• Fast scan 8 m at 10 micron

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Genome Variation Laboratory

• Contact Dr Michael James
• Michael.James_at_qimr.edu.au
• Sequenom MassARRAY Compact
• MALD-TOF MS analysis
• optimised for DNA fragment
• 2 x 384 samples per run
• Primer extension SNP
• genotyping
• Gene expression assays
• DNA methylation analysis
• iPLEX multiplexing assay

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Whole Genome Genotyping and Gene Expression
Analysis

• Illumina Beadstation
• Miniaturised arrays of assay beads

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Illumina Beadstation

• Infinium Whole Genome Genotyping
• high-multiplex assays
• Genotype gt100,000 SNP loci simultaneously
• Exon-centric content emphasis
• Whole Genome Gene Expression
• 50mer gene-specific oligo probes
• Contact Nick Hayward
• Nick.Hayward_at_qimr.edu.au
• Expression Profiling with DASL Assay
• ideal for paraffin-embedded samples
• Contact Georgia Trench
• Nick.Hayward_at_qimr.edu.au

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Protein Technology Platforms

• High-end technology platforms

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Digital Imaging

• MD Typhoon 8600
• Phosphor-, fluorimager
• Fuji LAS3000
• Chemiluminescence

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Surface Plasmon Resonance

• Biacore 2000
• Characterize protein
• interactions
• Kinetic analysis
• Binding constants
• Binding specificity

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Multi-Mode Microplate Reader

• BMG PolarStar
• 96/384 well
• Absorbance
• Fluorescence Polarization
• Fluorescence Intensity
• Time-Resolved Fluorescence
• Luminescence (flash and glow)

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Multi-Analyte Detection System

• Bio-Rad Bio-Plex multiplex analysis system
• simultaneous analysis of up to 100 different
  biomolecules in a single microplate well.
• rare or volume-limited samples (i.e. serum)
• fluorescently dyed microspheres, or beads
• flow cytometer with 2 lasers to measure
  biochemical reactions that occur on the surface
  of the beads

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Protein Discovery Centre

• Contact Prof Jeff Gorman
• Jeffrey.Gorman_at_qimr.edu.au
• Federal funding 5 M
• Mass spectrometry
• Robotic spot-picking
• Robotic liquid handling
• Database search engines

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Mass Spectrometry

• Bruker microFlex MALDI-TOF
• Bruker ultraFlex MALDI-TOF/TOF
• reflectron, MS/MS
• Bruker HCTUltra ion trap
• non-ergodic fragmentation
• Bruker micrOTOF Q
• Tandem Quadrupole
• Thermo Finnigan LTQ OrbiTrap
• hybrid FT, MSn

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Q-Gen

• Contact Ms Allison McLean
• Allison.McLean_at_qimr.edu.au
• Production of clinical material
• cGMP standard, TGA approved
• Fermentation
• Cell Therapy
• PC3 Clean rooms

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Q-Gen Fermentation Suite

• Fermentation
• Cell culture
• MAB production
• Purification
• Formulation and packaging

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Cell Therapy Labs

• Clean rooms
• Class 350
• PC2 and PC3
• Production of cellular therapy reagents