Temple de la Sagrada Familia, 1883-

1
NCI/ATC MICROARRAY FACILITY The Art of Building
and Using Microarrays
Temple de la Sagrada Familia, 1883-
Gaudí's unfinished dream
2
A Trip Down Molecular Biology Memory Lane
1868 -- Rules of Heredity 1941 52 -- DNA
is the Hereditary Material 1953 -- 3D
Structure of DNA 1965 -- First Gene Sequence,
tRNA 1973 -- Recombinant DNA Cloning 1977 --
DNA/Gene Sequencing 1985 -- PCR Amplification
of DNA, automated sequencing 1995 -- Gene
Expression Microarrays 2000 -- Sequence of
Human Genome! (almost)
3
THE EVOLUTIONARY TREE ARE MORE GENES
BETTER? RICE IS 1 SO FAR! Organism
of Genes
Genome Size in BP Rice 50,000
430,000,000 Human
gt30,000
gt3,000,000,000 Mouse 30,000
3,000,000,000 Nematode 19,000

100,000,000 Drosophila
13,600
180,000,000 Yeast 6,300

14,000,000 E. coli 4,000
4,600,000 HIV-1

9-10
9,750
4
COURTESY OF NIH/NHGRI
5
(No Transcript)
6
NCI/ATC Microarray Facility Goals

• To manufacture quality microarrays for complex
• gene expression analysis, SNP assays, CGH,
  etc.
• To provide up to date training, protocols and
• technical support for microarray users.
• To develop new microarray technologies and
• applications.
• To form research collaborations with
  investigators
• whenever possible.
• Print entire human gene complement on one or
• two slides.
• Etc., etc.

7

• THE MICROARRAY FACILITY
• Two GeneMachine 1 BioRobotics Arrayer
  capacity is
• approx. 200-400 slides a week. Frederick lab
  similar.
• Scanners 2-4 Axon, 1 Agilent, 1 PerkinElmer.
  Able to
• analyze most fluors in visible range.
• Genes 10K human and mouse sets. 20-30K sets
  will be
• started using oligonucleotide 50-70mer sets.
  Rat oligos are
• also available.
• Affymetrix Gene Chips complete setup with
  latest software
• available at ATC and several locations on
  campus. 30K set
• Microarray lab courses held at ATC once a
  month.

8
NCI Microarray Facility Team Ernest Kawasaki -
Facility Head David Petersen - Production
Manager Inna Dzekunova - Sr Res Assoc Jonathon
Paarlberg - Res Tech 2-3 TBD - Res
Assoc/Scientists Beverly Stalker - Lab
Manager Special Thanks to Pedro Jares Visiting
Scientist