Root exudate induces protein secretion from RKN

1
Identifying Secretion Proteins of Meloidogyne
incognita
Patrick Shih, Stephane Bella fiore, Zhouxin Shen
Advisor Steve Briggs
Root exudate induces protein secretion from RKN
Tomato root exudate and resorcinol induce
secretion of partially overlapping protein sets
Tomato exudate
A.
B.
13
5
Tomato exudate
97.8
1
19
Intact J2
79
Resorcinol
Intact J2
16.2
34
1,834
246
Resorcinol
Nematode database search reveals 2,226 proteins
from secretions plus whole M. incognita at J2
stage. The secretome incudes 392 proteins.
Summary
The secretome contains at least 711 proteins

• Previous studies of plant pathogenic nematodes
  have identified 10 secreted proteins. All 10 were
  observed in our dataset of 741 secreted proteins.
• Our secretome can be divided into three main
  categories
• Proteins involved in metabolism and protein
  degradation (50).
• Proteins that could be involved in the
  host-pathogen interaction (15-20).
• Other
• 3. Several proteins could have been acquired by
  horizontal gene transfer from plants and the
  question concerning their role in plant-pathogen
  interaction remains open.

NCBI nr Plant database 32 proteins, 72 peptides
RefSeq database 335
Nematode database 392
16
2,400 unique peptides revealed 711
proteins (Average of 3.4 peptides per protein)