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EP

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EP & TA Laboratories and Veterinary Diagnostics Ltd. 16 Phalirou Str. Ayios Pavlos 2362 ... EP & TA Laboratories ORGANOGRAM. Dr Pavlos Economides D.V.M. ... – PowerPoint PPT presentation

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Title: EP


1
EP TA Laboratories and Veterinary Diagnostics
Ltd
  • 16 Phalirou Str. Ayios Pavlos 2362
  • Nicosia Cyprus
  • Tel. No. 00357 22 444 230
  • Fax No. 00357 22 444 231
  • Email eptalab_at_gmail.com

2
EP TA Laboratories ORGANOGRAM
  • Dr Pavlos Economides D.V.M.-F.R.S.H
    Veterinarian-Bacteriologist Director
  • Dr Savvas Gennaris D.V.M.-Veterinarian
  • Dr Marios Christoforou DVM-Msc
    Veterinarian-Associate
  • Mr Tefkros Anaxagorou Quality Manager-Chief
    Technician
  • Mrs Alkisti Gennari Chemical Engineer
  • Mr Anaxagoras Anaxagorou Electrical
    Engineer-Laboratory Technician
  • Mr Anaxagorou Andreas - Sampling

3
EP TA Laboratories and Veterinary Diagnostics
Activities
  • VETERINARY DIAGNOSTICS
  • Microbiology
  • Parasitology
  • Serology
  • FOOD ANALYSIS
  • Bacteriology
  • Sensory Testing
  • Shelf Life Testing
  • Residues of inhibitory substances

4
TYPES OF SAMPLES EXAMINED
  • Animal Tissues Blood samples
  • Foods of animal origin
  • Meat and meat products
  • Milk and milk products
  • Fish and fish products
  • Animal feeds
  • Water

5
FOOD ESTABLISMENT HYGIENE
  • Water supply Bacteriological examinations-Chlori
    ne residues
  • Evaluation of working surface Hygiene Swab
    tests
  • Air samples - Bacteriology

6
METHODS FOR MICROBIOLOGICAL TESTING OF FOODS
  • Salmonellae ISO 6579-2002-Isolation
    Identification - Serotyping
  • Escherichia Coli ISO 21528-22004
  • Staphylococcus aureus ISO 6888-12003
  • Listeria monocytogenes - Isolation
    Identification ISO 11290-21998
  • Colony-count- enumeration of microorganisms in
    animal feeding stuffs ISO 48332003
  • Detection of Staphylococcal Enterotoxin in milk
    products ELISA Transia Kit

7
METHODS FOR MICROBIOLOGICAL EXAMINATIONS OF WATER
  • Detection and enumeration of Escherichia coli and
    Coliforms bacteria-Membrane filtration method ISO
    9308-1
  • Detection and enumeration of intestinal
    enterococci ISO 7899-2
  • Total colony count ISO 6222
  • Detection and enumeration of Faecal Coliforms ISO
    9308-1
  • Detection of Legionella spp ISO 11731

8
METHODS FOR MICROBIOLOGICAL TESTING OF SURFACES
AND AIR SAMPLES
  • SAMPLING
  • Rodac-contact plates
  • Hygicult Total Plate Count Agar
  • Hygicult E-Violet Red Bile Agar with addition of
    glucose
  • TESTING
  • Total Plate Count ISO 48332003
  • Detection and enumeration of Enterobacteriaceae
    ISO 21528-22004
  • Detection of Salmonella spp ISO 6579-2002
  • Detection and Enumeration of Listeria
    monocytogenes ISO 112901996

9
Bacteriological Examination
  • Cultures
  • Identification of Bacteria Biochemical testing
  • P.C.R.-Polymerase Chain Reaction
  • Immunofluorescence

10
Polymerase Chain Reaction Immunofluorescence
Examination
  • PCR
  • Legionella spp
  • Salmonellae spp
  • Listeria spp
  • Rickettsia
  • Coxiella
  • Leishmania
  • Bartonella
  • Immunofluorescence
  • Legionella spp
  • Rickettsia
  • Coxiella
  • Leishmania
  • Bartonella

11
SEROLOGICAL TESTING
  • Standard Agglutination Tests
  • Complement Fixation Test
  • ELISA Tests

12
ELISA TESTS
  • ELISA IN POULTRY
  • Infectious Bursal Disease IBD
  • Infectious Bronchitis Virus IBV
  • Newcastle Disease Virus NDV
  • Avian Pneumovirus TRT
  • Reovirus - REO
  • Mycoplasma gallisepticum - MG
  • ELISA IN PIGS
  • Reproductive and Respiratory Syndrome PRRS
  • Swine Influenza
  • Aujeszky Disease
  • Mycoplasma Hyopneumoniae
  • Porcine Parvovirus
  • Transmissible Gastroenteritis - TGE
  • Corona Virus

13
PARASITOLOGICAL TESTING
  • Microscopical examination
  • Parasites Egg Count by Floatation McMaster
  • Protozoa

14
CUSTOMERS
  • Number of Establishments
  • 78
  • Types of Establishments
  • Poultry Slaughter houses
  • Slaughter houses for pigs, sheep and goats
  • Food establishments
  • Meat Cutting Plants
  • Meat Products
  • Dairies
  • Butcheries
  • Hotels
  • Restaurants
  • Number of Farms
  • 43
  • Types of Farms
  • Dairy Farms
  • Pig Farms
  • Poultry Farms
  • Rabbit Farms
  • Pets-dogs-cats
  • Cage birds

15
SALMONELLAE SEROTYPES ISOLATED
  • List of most common Salmonellae serotypes
    isolated
  • Typhimurium
  • Braenderup
  • Brandenburg
  • Newport
  • Hadar
  • Derby
  • Anatum

16
LISTERIA SEROTYPES ISOLATED
  • List of most common Listeria serotypes isolated
  • Listeria monocytogenes
  • Listeria innocua
  • Listeria welshimeri

17
SURVEILLANCE FOR ZOONOSES
  • Brucellosis
  • Rickettsioses
  • Coxiella
  • Bartonellosis
  • Leishmaniosis
  • Ehrlichiosis

18
COLLABORATION WITH OTHER INSTITUTIONS
  • World Health Organization WHO network Salm-Surv
    as a General Institution Member for the GSS
    country Databank
  • University of Crete School of Medicine -
    Laboratory of Clinical Bacteriology-Parasitology-Z
    oonoses and Geographic Medicine
  • WHO Collaborating Center for Research and
    Training in Mediterranean Zoonoses Anogia -
    Crete
  • Royal Veterinary College London Department of
    Pathology of Infectious Diseases - UK

19
PARTICIPATION IN INTERLABORATORY TESTS
  • We participate in all Interlaboratory testing
    organized by the National Reference Laboratory
    for foods of Animal Origin
  • July 2004 Total Colony Count in milk samples
    (accuracy d-0,03)
  • October 2004 - Total Colony Count in milk samples
    (accuracy d-0,022)
  • September 2005 Detection of Salmonella and
    Listeria in cheese samples (accuracy 100)
  • May 2006 - Total Colony Count in milk samples
    (accuracy d-0,009)

20
APPROVAL - LICENCED
  • EP TA Laboratories and Veterinary Diagnostics
    is approved and licensed by
  • The Department of Veterinary Services of Cyprus
  • The Veterinary Council
  • The diagnosis tests of animal diseases, zoonotic
    agents and for the bacteriological examination of
    foods of animal origin including animal feeds. We
    are in the process for accreditation in
    accordance with the requirements of the ISO
    170252005 standard.
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