Response to BERAC Review

1
Response to BERAC Review

• Overview of JGI
• BERAC Recommendations
• Managing multi-lab environment
• Operation of user facility
• LSP program
• JGI Energy Portfolio

2
JGI Mission

• User facility providing DNA sequencing
  analysis in support of DOE missions in
  alternative energy, carbon cycling
  bioremediation.

www.jgi.doe.gov
3
Cost-effective Sequencing at JGI
FY06 capacity 30 billion basepairs
Cntr1
Cntr4
Cntr3
Cntr2
Cntr5
Cntr6
JGI
NIH data provided by Jane Peterson, NHGRI
4
Success of JGI User Programs
5
Primary JGI User Distribution
KEY REDAcademic BlueNational Laboratory
BlackIndustry
6
1st Annual JGI User Meeting
JGI User Meeting, April 2006 250 Users and
potential users Sequence-based science
talks Tours of JGI facility Poster sessions User
Committee formed
7
The JGI Partnership
JGI Director
LANL
LBNL
SHGC
PNNL
ORNL
PGF
8
BERAC RecommendationsOperations and Management-1

• Management of LBL/LLNL workforce
• Coordination of safety training tracking
• EHS MOU complete, waiting signatures
• LBL has 1º responsibility for EHS
• Cyber-security MOU
• Committee configured, meets this month

9
BERAC RecommendationsOperations and Management-2

• Succession planning for key JGI positions
• Hired production sequencing lead
• Senior managers identified seconds
• Seconds contribute to weekly mgmt meetings

10
BERAC RecommendationsOperations and Management-3

• Coordination of JGI partner lab tasks
• Weekly genome management conference call
• Monthly partners conference call
• Finishing in the future meeting
• Eukaryotic Genome Program summit

11
BERAC RecommendationsUser Facility
Proposals
Sequencing
Annotation
12
BEERAC RecommendationsUser Facility-1

• Managing the User Facility
• Peer review
• MGP and CSP programs/review groups merged
• Broadened geographical representation
• Electronic proposal submission review
• Letter-of-intent process
• User committee input on peer review committees

13
BEERAC RecommendationsUser Facility-2

• Managing the User Facility
• Formal project management
• Appointed project management lead
• Hired 2 new project managers
• Launched Global Project Tracking System
• End-of-project survey in draft form

14
BERAC RecommendationsUser Facility-3

• Managing the User Facility
• Improve large genome analysis throughput
• JAZZ assembly program overhauled
• 2 programmers hired into assembly group
• 3 people hired into annotation group

15
Lab Science Program

• LSP created by new JGI MOU
• Facilitate access to JGI for Natl. Lab
  investigators
• Fulfill programmatic sequencing needs of DOE
• Shape JGI products and services to meet needs of
  the Natl. Labs
• BERAC concerns
• Scientific vision for LSP
• Implementation

16
BERAC RecommendationsLSP Program

• LSP lead
• Appointed Jerry Tuskan, ORNL, Jan 06
• Focus on bioenergy
• LSP lead now reports to JGI Director
• Implementation
• Lab points-of-contact identified
• Small-scale sequencing program
• 14 proposals received, 10 approved to date
• Large-scale sequencing program
• Plant Genomes for Energy white paper
• Fungal Genomes for Energy white paper

17
Bioenergy the JGI
Corn based ethanol
Soy-based biodiesel
NSF/USDA/DOE funded project
LSP project
Transportation Fuels
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JGI and Bioenergy
Saccharification
Fermentation
Cellulose
Sugars
19
Auxin Regulation of Poplar Shape
90-day-old Populus cuttings
Enhanced radial growth of IAA16.3 transgenic
trees
IAA16.3
Control
20
New Cellulase Genes from Termite Gut
Cellulose
15 Endoglucanases
12 Exoglucanases
Cellobiose
H2O
P
Cellobiose phosphorylase
22 ?-glucosidase
P
Glc
Glc
Glc
Glc-1-P