Parasitology Laboratory Two: Kinetoplastida

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Parasitology Laboratory Two Kinetoplastida

• Alaine K. Knipes - January 22, 2008

with Figures from Roberts Janovy, Foundations
of Parasitology, 7th edition 2005
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Task 4 Various lifecycle stages of kinetoplastid
flagellates.
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Task 4
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Task 4
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Task 4
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Task 4
Trypomastigote
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Lifecycle Leishmania donovani
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Leishmania donovani E9
Intermediate host and Vector
Vertebrate (mammal) host tissue
In Phlebotomus Sand fly Vector Sand flies suck
blood of infected animal, ingesting amastigotes.
Parasites develop in midgut or hindgut of sand
fly, into promastigotes. Promastigote stage
parasites migrate to the esophagus and pharynx of
sandfly. Sand fly injects active promastigotes
into vertebrate host while taking a blood meal.
In the vertebrate host Parasite lives inside
macrophages of vertebrate blood, spleen,
liver.
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Leishmania donovani E9
Intermediate host and Vector
Vertebrate (mammal) host tissue
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Lifecycle Trypanosoma brucei gambiense
Trypanosoma brucei gambiense E12
Glossina (Tsetse fly)
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Trypanosoma brucei gambiense E12
In Glossina (Tsetse fly)
Glossina suck up parasites in when taking a blood
meal from an infected vertebrate host. The
parasites locate in the posterior section of the
insects midgut.
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Trypanosoma brucei gambiense E12
In Glossina (Tsetse fly)
The trypomastigotes migrate to the foregut, then
the esophagus and pharynx, and transform to
epimastigotes. The epimastigotes then undergo
asexual reproduction in the lumen of the tsetse
fly salivary glands. Finally transforming back
into metacyclic trypomastigotes.
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Trypanosoma brucei gambiense E12
In Glossina (Tsetse fly)
Finally, the infective trypomastigotes are
injected into the vertebrate host while the
tsetse takes a blood meal.
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Trypanosoma brucei gambiense E12
In vertebrate hosts
Once in the vertebrate host, the parasites
multiply as trypomastigotes in the blood and
lymph.
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Trypanosoma cruzi E10
Lifecycle Trypanosoma cruzi
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Trypanosoma cruzi E10
In Triatoma (reduviidae)
When reduviid bugs feed they often deficate on
the skin of their host. Their feces may contain
metacyclic trypanosomes, which gain entry into
the body of a vertebrate host through a bite,
through scratched skin, or, most often, through
mucous membranes that are rubbed with fingers
contaminated with insects feces. (Foundations,
p 71)
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Trypanosoma cruzi E10
In Vertebrate host
Amastigotes are likely to form pseudocysts in
tissues such as heart muscle (and sometimes
brain).
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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Trypanosoma cruzi E10
In Vertebrate host
Trypomastigotes will be in the host blood.
All Figures from Roberts Janovy, Foundations of
Parasitology, 7th edition 2005
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(No Transcript)
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For your notes

• Kinetoplast conspicious part of the
  mitochondrion in a trypanosome usually found
  near the kinetosome.
• Kinetosome centriole for from which the axoneme
  arises.
• Axoneme core of a cilium or flagellum,
  comprising microtubules.