Development of a Volatile Delivery System

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Development of a Volatile Delivery
System (Ongoing Research)
Matt Bennett
Dr. Mark E. Whalon Dept. of Entomology Andrea
Coombs Dept. of Entomology
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Background

• Robert J. Bartelt, USDA
• Journal of Chemical Ecology
• 1990
• 1997

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Objectives of Project

• Build Bartelts VDS
• Figure out how it works
• Make Improvements
• Research
• Bioassays
• Attractants
• Repellants

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What is it?

• Volatile Mixes
• Chemicals
• Pheromones
• Other

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Definitions

• Volatile Chemicals
• a compound released from a plant that can either
  attract, repel, or otherwise influence insect
  behavior.
• Pheromones
• a chemical produced by insects that illicits
  behaviors from other insects of the same species.

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Why?

• Monetary Losses
• Realistic Approach
• Bioassays With Less Hassle
• FQPA
• Model

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Insect Studied

• Plum Curculio
• Conotrachelus nenuphar
• Weevil
• Family Coleoptera
• Plants (Roseaceae)
• Cherry
• Hawthorn
• Apple

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Plant Studied

• Apple
• Apple Buds
• Crescent Scars
• Larvae

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How it Works

• Zero Chromatographic Air
• gasses, hydrocarbons, moisture
• Hydrocarbon Trap
• organic compounds
• Air Flow Regulators
• Make up air

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How it Works

• Zero Chromatographic Air
• gasses, hydrocarbons, moisture
• Hydrocarbon Trap
• organic compounds
• Air Flow Regulators
• Make up air
• Normal Flow

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How it Works (contd)

• Chambers
• Volatile
• Pheromone

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How it Works (contd)

• Splitter System
• Restrictor Needles
• Volatile mix

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How it Works (contd)

• Backpressure Created

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How it Works (contd)

• Backpressure Created
• Manometers

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How it Works (contd)

• Backpressure Created
• Manometers
• Regulation Valves

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How it Works (contd)

• GC Port
• Quantitative Analysis

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How it Works (contd)

• GC Port
• Quantitative Analysis
• Exit to Insect Chamber
• Heated
• Air Pulled Through

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Advantages

• Versatility
• Options
• Ratios
• Closer to Reality

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Improvements (thus far)

• Larger Size
• Easier to Work With
• More Volatiles
• SPME Port
• GC Analysis
• Insect Chambers
• Y-chamber

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Acknowledgements

• Dr. Mark E. Whalon
• Department of Entomology, MSU
• Andrea B. Coombs
• Department of Entomology, MSU
• Robert J. Bartelt
• USDA/ARS/NCAUR
• Kevin W. Youngs