Micro for Nursing Lab

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Micro for Nursing Lab

• Dr. Julie Huggins

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WEB SITE

• For all course information go to
• http//www.clt.astate.edu/jhuggins

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Office hours and misc.

• Office is LSW 550
• Phone-972-3555
• Office hours M,T, Th, F 12-100 and Wed
  100-200
• TA-Michel or Josh

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Required materials

• Sharpie (black)
• Microbiology Laboratory Theory and Application
  (BRIEF ed.) by Leboffe and Pierce, Morton
  Publishers
• Always read your assignment before class.

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Grading

• 4 pop quizzes (25 points each)
• 1 Pet report (100 points)-unknown identification
• 1 lab final (100 points)
• 1 midterm (written) 50 points
• 1 technique grade on gram stain (50 points)
• TOTAL-400 points

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Attendance and makeup tests

• Attendance is mandatory if you want a good grade
• Anyone who misses the lab practical (final) will
  get an oral exam
• Make up tests are harder!
• If you dont come to class you get behind on your
  lab work and confusion is the result!
• If you dont come for a scheduled make up test
  without calling to cancel, you wont get a second
  chance.

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Classroom policy

• Absolutely NO eating or drinking in class
• We are working with live, potentially pathogenic
  bacteria in lab
• Be careful with the equipment, glassware, flame,
  cultures, microscopes
• Dispose of paper products in waste basket
• Dispose of contaminated materials and old
  cultures in Biohazard area (buckets)

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Rules

• Read introduction p. 1-5 (there will be a quiz
  next week over the syllabus and these pages)
• Turn off Bunsen burners when not in use and keep
  them in the middle of the table when in use.
• Wear gloves when staining or handling organisms
• Tie hair back, dress appropriately for lab days
• Label cultures with sharpie

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Rules cont.

• Contact instructor immediately if you spill or
  break something
• Do not walk around with an open culture dish or
  tube
• Close tubes and dishes quickly (the longer they
  remain open the more likely it is your culture
  will get contaminated.)
• Dispose of used bacteria in biohazard area
• Put everything away after use

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Lab daily schedule

• Wash hands and station when you first come in
• Roll call
• Introduction by TA or Instructor
• Review last weeks exercise/note results
• Receive cultures/media and inoculate
• Clean equipment (microscope), dispose of used
  cultures, clean table, wash hands