Target Selection Helicobacter Pylori

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Target Selection -Helicobacter Pylori
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Target Selection -Helicobacter Pylori
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Classification and target
Sequence similarities (BLAST)
Structural identity (PDB)
Ultimate Targets
No
No
Less identity Less/No positive
Immediate Targets
No
Less identity Less/No positive and Less
identity More positive
Immediate Preferred Targets
lt30 sequence Similarities
gt30 sequence Similarity
More identity More positive
Targets for Modeling
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Record 1 (w/o cystine)
BINDING OF EXOGENOUSLY ADDED CARBON MONOXIDE AT
THE ACTIVE SITE OF THE IRON-ONLY HYDROGENASE
(CPI) FROM CLOSTRIDIUM PASTEURIANUM BIOCHEMISTRY
V. 38 12969 1999
Stable structure?
Helicobacter pylori is an anaerobic
bacteria -requires bacteria for its life cycle
Role in Carbon monoxide binding?
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Record 10 (w/o cystine)
Crystal Structure Of Type 2 Ribonuclease H From
Hyperthermophilic Archaeon, Thermococcus
Kodakaraensis Kod1. PROTEIN SCI. V. 10 707 2001
Heat Shock protein?
Signal recognition?
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Record 22 (w/o cystine)
Crystal Structure Of Wild Type Turkey Delta 1
Crystallin (Eye Lens Protein) BIOCHEMISTRY V. 40
2732 2001
Unknown function?
Strong propensity to form helix?
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Record 1 (with one cystine )
Structure at 2.3 A resolution of the cytochrome
bc(1) complex from the yeast Saccharomyces
cerevisiae co-crystallized with an antibody Fv
fragment. Structure Fold Des 2000 Jun
158(6)669-84
Unknown function
Strong propensity to form helix?
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Record 3 (with one cystine )
Large-scale preparation, purification, and
crystallization of UDP-N acetylmuramoyl-L-alanine
D-glutamate ligase from Escherichia coli
Protein Expr. Purif. 13 (1), 23-29 (1998)
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Record 3 (with one cystine )
Crystal structure of cleaved bovine antithrombin
III at 3.2 A resolution J. Mol. Biol. 232 (1),
223-241 (1993)
Anti protease? Bacterial Ligase?
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Record 7 (with one cystine )
New insight into the catalytic mechanism of
chorismate mutases from structural studies. Chem
Biol 1995 Apr2(4)195-203
biosynthesis of aromatic amino acids?
Potential Target??? Used in preparation of
essential amino acids??
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Record 25 (with TWO cystines )
Crystal structure of the SarR protein from
Staphylococcus aureus Proc. Natl. Acad. Sci.
U.S.A. 98 (12), 6877-6882 (2001)
DNA binding protein - receptor and activator
Two cystines 12 residues away
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Record 45 (with TWO cystines )
Three-dimensional structure of interleukin-2
Science 238 (4834), 1707-1709 (1987)
Very stable protein, disulfide cross linked??
Two cystines 27 residues away
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THE END
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Back to Slide 1
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Back to Slide 1
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Record 1 (w/o cystine)
(Back to Record 1 w/o Cys)
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Record 10 (w/o cystine)
Record 10 (w/o cystine)
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Record 22 (w/o cystine)
Record 22 (w/o cystine)
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Record 1 (with one cystine )
Record 1 (with one cystine)
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Record 3 (with one cystine )
Record 3 (with one cystine)
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Record 7 (with one cystine )
Record 7 (with one cystine)
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Record 25 (with TWO cystines )
Record 25 (with two cystines)
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Record 45 (with TWO cystines )
Record 45 (with two cystines)