The Human Metabolome Project

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The Human Metabolome Project

• Lori Querengesser
• Genome Prairie
• Dept. of Computing Science, University of Alberta
• loriq_at_ualberta.ca
• www.metabolomics.ca

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Human Metabolome Project

• What is it?
• Whos involved?
• Whats the HMDB and the HML?
• How can it help?

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Human Metabolome Project

• 7.5 million Genome Canada Project launched in
  Jan. 2005
• Mandate to quantify (normal and abnormal ranges)
  and identify all metabolites in urine, CSF,
  plasma and WBCs
• Make all data freely and electronically
  accessible (HMDB)
• Make all cmpds publicly available (HML)

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Human Metabolome Project

• Purpose is to facilitate Metabolomics
• Objective is to improve
• Disease identification, prognosis monitoring
• Drug metabolism and toxicology
• Linkage between metabolome genome
• Development of software for metabolomics

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Why 1 mM?

• Equals 200 ng/mL
• Limit of detection by NMR
• Limit of facile isolation/separation by many
  analytical methods
• Excludes environmental pollutants
• Most IEM indicators and other disease indicators
  have concentrations gt1 mM
• Need to draw the line somewhere

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Human Metabolome Project

• What is it?
• Whos involved?
• Whats the HMDB and the HML?
• How can it help?

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David Wishart Comp. Sci. U of Alberta Proj. Leader
Brian Sykes Biochemistry U of Alberta NMR spect.
Russ Greiner Comp. Sci. U of Alberta Bioinformatic
s
Hans Vogel Biochemistry U of Calgary NMR spect.
Fiona Bamforth Clin. Chemistry U of
Alberta Sample Acq.
Derrick Clive Chemistry U of Alberta Synthesis
Liang Li Chemistry. U of Alberta MS/Separation
Mike Ellison Biochemistry U of Alberta MS/Separati
on.
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Human Metabolome Project

• What is it?
• Whos involved?
• Whats the HMDB and the HML?
• How can it help?

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The HMDB the HML

• HML is the Human Metabolome Library
• Repository of chemical samples for public
  redistribution
• Includes purchased, isolated synthesized cmpds
  (many unique or rare cmpds)

• HMDB is the public face of the HMP
• Freely web-accessible database providing detailed
  information on metabolites, chemistry, enzymes,
  diseases, pathways
• Links metabolome to genome

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Human Metabolome Database
www.hmdb.ca
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The HMDB MetaboCard
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The Human Metabolome Library

• 10 mg 10 g of 1400 human metabolites
• may be purchased for use as references or
  standards by commercial or clinical testing labs
• may be obtained free of charge for University and
  Hospital researchers interested in collaborative
  research or those who will acknowledge the use
  and source of the compounds in publications

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The HML Allows One To

• Access rare or unusual metabolites as references
  or standards for MS, HPLC, GC-MS or NMR analyses
• Compare newly isolated compounds with known cmpds
  (saves on reinventing the wheel)
• Use these compounds as precursors to synthesize
  new metabolites

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HML HMDB Status

• 825 compounds IDd to date
• 600 compounds with sources
• 325 compounds purchased
• 100 compounds fully characterized and archived in
  HML
• 800 partial entries in the HMDB
• Full set of 235 HMDB expected July 1
• Text based and will be continuously updated as we
  progress

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Metabolomics LIMS System
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Metabolomics LIMS System
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Metabolomics LIMS System
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Human Metabolome Project

• What is it?
• Whos involved?
• Whats the HMDB and the HML?
• How can it help?

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We Need You!

• Always looking for collaborations
• We have 8 million in equipment (NMR, HPLC,
  FT-ICR, QTOFs, etc) ready and waiting for samples
  to be analyzed
• We have 4 full-time programmers to develop
  software you may want or need
• Existing collaborations on cachexia, prostate
  cancer, pneumonia, obesity

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Thanks to...