Use of dialysis to separate small and large molecules - PowerPoint PPT Presentation

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Use of dialysis to separate small and large molecules

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Molecular mass determination by gel filtration chromatography ... Logarithmic relationship between the molecular mass of a protein and its ... – PowerPoint PPT presentation

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Title: Use of dialysis to separate small and large molecules


1
Use of dialysis to separate small and large
molecules
2
Gel filtration chromatography
3
Molecular mass determination by gel filtration
chromatography
4
Some commonly used gel filtration materials
5
Ion-exchange chromatography with stepwise elution
6
Device for generating a linear concentration
gradient
7
Molecular formulas of cellulose-based ion
exchangers
8
Some biochemically useful ion exchangers
9
Affinity chromatography
10
Covalent linking of ligand to agarose
11
Derivatization of epoxy-activated agarose
12
Purification of staphylococcal nuclease by
affinity chromatography on bisphosphothymidine-lin
ked agarose
13
Experimental arrangement for paper chromatography
14
Two-dimensional paper chromatography
15
Paper electrophoresis
16
Apparatus for slab gel electrophoresis
17
Diagram of a disc electrophoresis apparatus
18
Polymerization of acrylamide and
N,N-methylenebisacrylamide to form a
cross-linked polyacryl-amide gel
19
SDS-PAGE
20
Logarithmic relationship between the molecular
mass of a protein and its relative
electrophoretic mobility in SDS-PAGE
21
General formula of the ampholytes used in
isoelectric focusing
22
Isoelectric points of several common proteins
23
Purification of rat liver glucokinase
24
END
25
An enzyme-linked immunosorbent assay (ELISA)
26
Solubilities of several proteins in ammonium
sulfate solutions
27
Solubility of caboxy-hemoglobin at is isoelectric
point as a function of ionic strength and ion type
28
Solubility of b-lactoglobin as a function of pH
at several NaCl concentrations
29
Protein crystals
30
Agarose gel electrophoretogram of a double
helical DNA
31
Pulsed-field gel electrophoresis (PFGE) of a set
of different sized bacteriophage DNAs
32
Detection of proteins by immunoblotting
33
Two-dimensional (2D) gel electrophoresis
34
Sedimentation coefficients in Svedbergs (S) for
some biological materials
35
A selection of preparative ultracentrifuge rotors
36
Zonal ultracentrifugation
37
Equilibrium density distribution of a CsCl
solution in an ultracentrifuge spinning at 39,460
rpm
38
Isopycnic ultracentrifugation
39
Physical constants of some proteins
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