Micro-Spherical Heart Pump Powered by Cardiomyocytes

1
Micro-Spherical Heart Pump Powered by
Cardiomyocytes
Yo Tanaka1, Kae Sato1, Tatsuya Shimizu2, Masayuki
Yamato2, Teruo Okano2, Takehiko Kitamori1
1The University of Tokyo, Bunkyo, Tokyo,
113-8656, Japan 2Tokyo Womens Medical
University, Shinjuku, Tokyo, 162-8666, Japan
Ref µTAS2006

• ??? 9633584 ???

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Outline

• Introduction
• Other Micro-Pump
• This Groups Micro-Pump
• Materials and Methods
• Results and Discussion
• Conclusions
• References

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Introduction Other Micro-Pump
Electric Power
Electric Powerless
4
Introduction This Groups Micro-Pump
5
Materials and Methods

• A hole (600 µm in diameter) in the center of
  sugar ball and edible silver was detached.

• Teflon capillary (200 µm in inside and 400 µm in
  outside diameters) was threaded through the hole
  and molten glucose by using a hotplate at 150 ?
  was applied around the hole.

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Materials and Methods

• About 1 mL of PDMS prepolymer solidified at 100 ?
  for 1 hour above a hotplate under rotation (22
  rpm).

• Drawing and insertion of capillaries, and the two
  capillaries were attached to sphere using epoxy
  glue.

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Materials and Methods

• One capillary was connected to a syringe pump and
  dissolving sugar ball in water.

• A hollow sphere (about 5 mm in diameter, 250 µm
  in thickness) with connected capillaries was
  fabricated.

• The PDMS sphere was sterilized and immersed for 1
  h in 50 µg mL-1 fibronectin solution in PBS at 37
  ? to promote cardiomyocyte attachment.

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Results and Discussion

• A cultured cardiomyocyte sheet produced periodic
  contractile-expansion motion of the PDMS micro
  spherical heart.
• Spherical polystyrene tracking particles (1 µm
  diameter) were dispersed in cell culture medium
  within the capillaries.

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Results and Discussion
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Conclusions

• New fabrication methods to create a novel micro
  spherical heart-like pump prototype.
• Regular fluid motion in a capillary connected to
  the hollow pumping sphere, with the device
  working continuously over 5 days.
• This device is a fully integrated, wireless
  mechanochemical converter, driven with only
  chemical energy input from culture milieu.

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Conclusions

• External control of both fluid motion and
  mechanical performance of the bio-actuator is
  possible using culture temperature.
• Possible applications
• as an electric powerless bio-actuator to drive
  fluids in implanted micro-chemical or biochemical
  medical implant devices.
• as a component of a cardiovascular circulatory
  system micro-model to study mechanisms of
  circulatory physiology, pathology, and
  developmental biology.

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References

1. Y. Tanaka, K. Morishima, T. Shimizu, A. Kikuchi,
   M. Yamato, T. Okano, T. Kitamori, Lab Chip, 6,
   362-368, (2006).
2. Y. Tanaka, K. Sato, T. Shimizu, M. Yamato, T.
   Okano and T. Kitamori, Lab Chip, 2007, 7,
   207212.
3. Y. Tanaka, K. Sato, T. Shimizu, M. Yamato, T.
   Okano, Ichiro Manabe, Ryozo Nagai and T.
   Kitamori, Lab Chip, 2008, 8, 58-61.
4. Jungyul Park, Il Chaek Kim, Jeongeun Baek, Misun
   Cha, Jinseok Kim, Sukho Park, Junghoon Lee and
   Byungkyu Kime, Lab Chip, 2007, 7, 13671370