INSTITUTE OF CHEMISTRY

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INSTITUTE OF CHEMISTRY DEPARTMENT OF STRUCTURE
AND FUNCTION OF SACCHARIDES
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Staff 6 scientists 2
technicians 2 PhD students Main
instruments used in research 3 computers
Quantum Cube 1 computer Silicon Graphics
Origin 200 2 Linux clusters High-resolut
ion NMR spectrometer 300 MHz Bruker
DPX 600 MHz Varian Unity Mass
spectrometer Finnigan SSQ 710
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Projects International Projects within
5th and 6th Framework Program EU Mitzutani
grant Slovak agencies APVV and VEGA
projects International collaboration Ita
ly G.Ronzoni Institute for Chemical and
Biochemical Research, Milan Politechnico
di Milano, Milan Germany Institute for
Chemistry, Medical University Lübeck,
Lübeck Spain Centro de Investigaciones
Biológicas, CSIC, Madrid France CERMAV, CNRS,
Grenoble SEESIB, University B. Pascal-CNRS,
Aubiere INRA, Theix, Clermonf-Ferrand Canada
Trend University, Petersborough USA NIH,
Bethesda Czech Republic Institute of Analytical
Chemistry, CAS, Brno
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Main research areas 1. Structure of saccharides
in solution Saccharides investigated glycosam
inoglycans, heparin-like oligo- and
polysaccharides 2-C-branched saccharide
derivatives nucleosides, nucleotides, uronic
acids plant polysaccharides,
lipolysaccharides Methodology 1D and 2D NMR
methods in solution (COSY,HSQC, HMBC,
NOESY,..) MALDI, ES, GC-MS Quantum chemical
and DFT calculations, (Jaguar, ADF,
Gaussian) 2. Dynamics of saccharides in
solution Saccharides investigated
glycosaminoglycan oligosaccharides and
polysaccharides Methodology 2D NMR
heteronuclear relaxation data (T1, T2, NOE)
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3. Degradation of plant polysaccharides Saccharid
es investigated plant polysaccharides
Methodology 2D NMR (COSY, HSQC,
HMBC) solid-state NMR 4. Intermolecular
interactions a) protein-carbohydrate
interactions Systems investigated
interaction of oligosaccharides with
glycosylhydrolases, glycosyltransferases,
growth factors, heparanase,
antithrombin Methodology Quantum chemical, DFT,
QM-MM methods (ADF, Gaussian, Jaguar) 2D
NMR (transferred NOESY, STD) b) transition
metal complexes of carbohydrates Systems
investigated Mo(VI) complexes with saccharides
derivatives Methodology 2D NMR (COSY, HSQC,
HMBC) solid-state NMR, X-ray
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6. Development of new theoretical
methods Methodology New quantum chemical
methods for analysis of 3D saccharide
structures