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TESTING AND SURVEILLANCE
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HIV PROTEINS ...analysis of the proteins
demands mass production and purification Luc
Montagnier 1997
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Montagnier 1983 Material at 1.16 gm/ml Purified
virus 3 proteins react with patient
serum p24/25 HIV No EM
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I REPEAT, WE DID NOT PURIFY NO PARTICLES
TYPICAL OF RETROVIRUSES IN THE PURIFIED
VIRUS Luc Montagnier Pasteur Institute
Interview July 1997
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THE HIV PROTEINS p41/120/160 MONTAGNIER 1983,
1997 p41 PROTEIN IS CELLULAR ACTIN Arthur 1992
Orentas, 1993 Pearce-Pratt 1994 Sasaki 1995
Choudhury 1996 ACTIN PRESENT IN PURE HIV
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THE HIV PROTEINS p41/p120/p160 Pinter 1989 J.
Virology 63 2674-79 p160, p120 IN HIV WB
OLIGOMERS OF p41 Confusion over the
identification of these bands has resulted in
incorrect conclusionssome clinical specimens may
been identified erroneously as seropositive
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THE HIV PROTEINS p24 MORTIMER 1992 p24
NON-SPECIFIC SCHUPBACH 1992 p24 IN 49/60 (82) of
"presumably uninfected but serologically
indeterminate and 5/5 seronegative blood
donors Agbalika 1992 Vincent 1993 HIGHEST
LEVELS OF p24 IN NON-INFECTED ORGAN TRANSPLANT
RECIPIENTS
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THE HIV PROTEINS p32 Henderson1987 studied
the p30-32 and p34-36 of "HIV purified by double
banding" in sucrose density gradients. Comparison
with the amino-acid sequences of these proteins
with Class II histocompatability DR proteins
proved that "the DR alpha and beta chains
appeared to be identical to the p34-36 and p30-32
proteins respectively Cellular origin also
acknowledged by other HIV experts such as Arthur
1995.
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THE HIV PROTEINS- p17/18 Stricker 1987 Sera
from AIDS patients bind to a p18 protein bind to
mitogenically stimulated but uninfected in
uninfected lymphocytes Chassagne 1986 MCA to
"HIV" p18 reacts with dendritic cells in the
lymphatic tissues of a variety of patients with a
number of non-AIDS related diseases Parravcini
1988 the "same pattern reactivity was present in
normal tissue taken from uninfected individuals
as in those taken from HIV positive
subjects Faulk 1991 MCA to HIV proves p17
present in normal, uninfected human placenta
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HIV PROTEINS IN NORMAL HUMAN PLACENTA
p18/p24/p120 Faulk and Labarrere
1991 Immunocytochemical reactivity using poly-
and monoclonal antibodies Placentae from 25
normal term pregnancies were collected by vaginal
delivery...Antigens gp120 and p17 were identified
in normal chorionic villiAntigen p24in villous
mesenchymal cells...localized to HLA-DR positive
cells
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WHY THE ANTIBODY REACTIONS? Infectious agents
(inc. mycobact, fungi) Autoantibodies (many inc.
actin myosin) Non-specific and cross reacting
antibodies ?HIV
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GOLD STANDARD HIV ITSELF HIV ISOLATION
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HIV ISOLATION PROBLEMATIC RT (inc.
mitochondria) PARTICLES ANTIGEN/ANTIBODY
REACTIONS p24 ALL NON-SPECIFIC
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NIAID Web site Currently available HIV antibody
tests are extraordinarily accurate, both in terms
of sensitivity (the ability of the test to give a
positive finding when a subject is truly
HIV-infected) and specificity (the ability of the
test to give a negative finding when a subject is
truly HIV-free).
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Assay Name (Manufacturer) Global
Panel Sensitivity SpecificityDetect HIV 1
II 100 97.4 Cambridge 99.6 99.7 Abbott
3rd generation 100 100 HIV
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The panel included 332 HIV negative specimens and
203 sera positive for HIV-1 and 60 positive for
HIV-2 specimens
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GALLO JAMA 1985
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NO RISK HOSPITAL PATIENTS St. Louis 1988
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BURKE NEJM 1988 1.2 MILLION/135,187 1.4 and
0.148 per 1000 HEALTHY MILITARY RECRUITS
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BURKE HIV POSITIVE 2E
2WB HIV NEGATIVE NOT
ABOVE TRULY INFECTED 2E
2WB4XWB NOT TRULY INFECTED NOT ABOVE
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THE DE FACTO HIV GOLD STANDARDS THE CLINICAL
SYNDROME LOW RISK/GOOD HEALTH REPEATING THE TEST
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NIAID Web site Currently available HIV antibody
tests are extraordinarily accurate, both in terms
of sensitivity (the ability of the test to give a
positive finding when a subject is truly
HIV-infected) and specificity (the ability of the
test to give a negative finding when a subject is
truly HIV-free).
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At present there is no recognized standard for
establishing the presence or absence of HIV-1
antibody in human blood Abbott
Laboratories 1988, 1998
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The real purpose of scientific method is to make
sure Nature hasn't misled you into thinking
something you don't actually know... One logical
slip and an entire scientific edifice comes
tumbling down. One false deduction about the
machine and you can get hung up indefinitely.
Robert Pirsig, Zen and the Art of Motorcycle
Maintenance
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