Reconstitution of Dryvax

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Reconstitution of Dryvax Smallpox Vaccine

• As taken from the Package Insert,
• Dec. 2002

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Preparation of Vaccine

• Diluent and instructions for reconstitution will
  be supplied with vaccine.
• Vaccine may be used for 60 days following
  reconstitution.
• Vaccine must be reconstituted by a pharmacist,
  pharmacy technician or nurse.

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Glove Both Hands

• Prior to Opening Vaccine Vial

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Prior to Opening Vaccine Vial

• Record date of reconstitution on the bottle prior
  to performing the reconstitution.

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1. Lift up tab of aluminum seal on vaccine vial.
DO NOT BREAK OFF OR TEAR DOWN TAB.
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2. Wipe off vial stopper with an alcohol sponge
and allow to dry.
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3. Place vaccine vial upright on a hard, flat
surface. Insert a sterile 21 gauge or smaller
needle into the rubber stopper to releasethe
vacuum from the vaccine vial. Discard the needle
in biohazard waste container.
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• 4. To reduce viscosity of cold diluent, warm by
  holding diluent-cartridge in palm of hand for a
  few minutes.

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• 5. Peel open the needle package (provided with
  the kit) and aseptically remove the needle

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6. Remove rubber cover from end of the diluent
syringe.
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7. With a twisting motion, aseptically attach
the vented needle to the hub of the diluent
syringe.
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8. Remove protective cover from the vented
needle and expel the air from the diluent syringe.
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9. Aseptically insert the needle through the
rubber stopper into the vaccine vial up to the
first hub.
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10. Depress the plunger to ensure the entire
volume of diluent is delivered into the vial.
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• 11. Withdraw diluent syringe/vented needle and
  discard in biohazard waste container.

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• 12. Allow vaccine vial to stand undisturbed for
  3 to 5 minutes. Then if necessary, swirl vial
  gently to effect complete reconstitution. The
  vaccine may have a yellow tint and appear more
  viscous which is within normal limits for the
  vaccine.

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• 13. Place small piece of cardboard between the
  vaccine vial and the cold pack. Do not place
  vaccine vial directly on the cold pack.

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Storage

• If the vaccine is to be stored for subsequent
  use, re-stopper the vial and store at 2-8 degrees
  C (35 46 degrees F.)
• The vaccine may be stored for 60 days after
  reconstitution.
• The vial of vaccine can be kept out at room
  temperature during vaccine clinic.

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Security

• The vaccine will be shipped to the clinics under
  armed guard.
• The vaccine will be stored at either the clinic
  site or at the Health Department when clinic
  operations are not underway and with security
  present.

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Security

• Security personnel will be responsible for
  monitoring the temperature of the refrigerators
  where the vaccine will be stored.
• Daily inventory will be performed and compared
  with previous inventories and reports of doses
  given at the clinic. Any discrepancies will be
  addressed upon discovery.