Hoods And Caging Used For Small Animal Containment

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Hoods And Caging Used For Small Animal Containment

• By Kevin Theis of
• D.A.I. Scientific Equipment

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Engineering Solutions to Air Contamination

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Airflow Schematic of Fume Hood
Offers Personnel Protection Only
ChemGard
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Airflow Schematic of Horizontal Flow Clean Bench
Offers Product Protection Only
EdgeGard
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Airflow Schematic of Biological Safety Cabinet
Offers Product, Personnel and Environmental
Protection
SterilGard III, Class II A2
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Classifications of Biological Safety Cabinets

• Class I Hood Only personnel protection
• Class II A1 70 recirculation/30 exhaust. 75
  FPM at front of hood
• Class II A2 70 recirculation/30 exhaust. 100
  FPM at front of hood
• Class II B1 40 recirculation/60 exhaust. 100
  FPM at front of hood
• Class II B2 100 exhaust. 100 FPM at front of
  hood

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Airflow of A1 cabinet
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Airflow of A2 cabinet
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Airflow of a B1 cabinet
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Airflow of B2 Cabinet
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Cage Changing Hoods
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Clean Bench

• 100 product protection
• Open area is easy to work in
• No allergen control
• Not that popular anymore

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Double Sided Hood

• Easy to Move
• Provides 100 product protection
• Limited personnel protection
• Adjustable Height
• 12 Opening

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Single Sided Hood

• Looks like BSC but only provides limited
  personnel protection
• 14 Opening
• More vertical space than double sided hood
• Can be used on stand or on lift

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Class II A2 Hood

• Provides Class II Protection
• 12 Opening
• Pre-Filters
• Adjustable Height

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Modified Class II A2 Hoods
Waste Dump
Necropsy
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Procedure Room Hoods

• Usually these hoods are used with anesthetics
• Because of vapor issue these hoods are a B1 or B2
  type.
• B1 has become more popular because its easier on
  the exhaust system

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Animal Receiving Hoods

• Located where animals first arrive
• Two Class II A2 hoods connected by a pass-thru

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Placement of BSC in Labs

• Keep away from all other sources of airflow
• opening doors and windows
• personnel traffic
• supply air diffuser
• other lab equipment
• Cross Draft Study
• personnel and product protection become
  compromised as cross drafts approach intake
  velocity

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Certification

• Once per year is required
• NSF offers listings on website
• www.nsf.org

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Reference Material
http//www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4toc.h
tm
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Caging in the Animal Area

• Static Cages
• Forced Air Racks
• Exhaust Ventilated Racks

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Static Cages

• Advantages
• Protect the animals
• Disadvantages
• Control of cages temperature and humidity is
  impossible causing animal stress
• Exposure to contaminants (animals, users, and
  environment)
• Labor Intensive due to frequent bedding changes

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Forced Air Ventilated Racks
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Forced Air Ventilated Racks

• Advantages
• Protect the animals
• Increase the number of animals per room
• Extend bedding changes (25-29 ppm NH3 after 2
  weeks)
• Reduce exposure to contaminants for animals,
  users, and environment

• Disadvantages
• Create animal stress (turbulent air, poor air
  quality, noise, vibration)
• Introduce fan heat to room
• Need electric outlets, sensors, alarms, emergency
  power, and redundancies
• Increase hardware, HVAC requirements/maintenance
  and utility costs
• Breeding may decrease

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Exhaust Ventilated CagingTHE M.I.C.E. SYSTEM BY
ANIMAL CARE SYSTEMS

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How it Works
- HVAC-Assist 20 Total-Volume ACH -
Venting Cleaner Environment -
Thermodynamics Fail-Safe
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(No Transcript)
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Airflow
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Micro Environmental Conditions
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Closed-System Design and Vent Filtration
Canard, G., Veillet, F., and P. Hardy. CRL, Iffa
Credo, Lyon, France
MicroIsolator Cage Reemay 2024 M.I.C.E. Cage
Vent Filter Unit 100 Collection Efficiency
at 0.3 µ (At Low-Pressure Low-Velocity,
it is as efficient as HEPA-filter)
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OptiMICE

• Space Saving Design
• Rack 33w x 39d x 78h and requires only 40 cfm
• 100 Cages
• 5.6 cages per sq. ft versus 2 cages per sq. ft on
  traditional racks
• Rotating design allows units to be closer to
  walls and other racks

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Space Saving
Room 10 x 17 420 cages versus 800 cages
with OptiMICE
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Exhaust Ventilated Caging

• Advantages
• Protect the animals, their users, and the
  environment
• Increase the number of animals per room
• Extend bedding changes (1-4 ppm NH3 after 3
  weeks)
• Reduce room hardware only 1 flex duct/rack (no
  fans, power cords and outlets)
• Eliminate maintenance, service, and calibration
  of fan and filter units
• Reduce HVAC requirements by lack of fans animal
  heat/odor loads
• Low total construction / renovation cost per
  square foot of animal facility
• Better environment for the animals better
  breeding performance.
• Disadvantages
• Rack is ducted to exhaust system

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Website Contact Information

• www.bakerco.com
• www.animalcaresystems.com
• Local Representation by
• D.A.I. Scientific Equipment
• Kevin Theis
• 800-816-8388 Ext. 12
• kevin.theis_at_daiscientific.com
• www.daiscientific.com