Clinical Microscopy

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Clinical Microscopy

• The Microscope

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Illuminator

• Halogen Bulb
• Usually controlled by a rheostat (knob that
  controls the intensity).

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Condenser

• Compound lens system that focuses the light from
  the illuminator onto the specimen.
• Gathers a large amount of light and condenses it
  into a small area.

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Aperture Diaphragm

• Lever on the front of the condenser that allows
  you to control the amount of light leaving the
  condenser.
• Opens and closes like the iris of you eye.

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Stage

• Simply the place where the slide sits.
• This area becomes very dirty. Keep it clean.

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Slide Holder

• Mechanical device that holds the slide in place
  on the stage. Where ever the stage goes the slide
  goes!

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Objectives

• Traditionally come in four flavors in the lab
• 10x, 40x, 50x, and 100x
• Magnify the image

• 50x and 100x require immersion oil.
• The oil bends light waves so more light enters
  the objective

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Oculars

• Usually have a magnification power of 10x.
• Form the final image.
• Total Magnification equals the oculars time the
  objectives. (40x times 10x 400)

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Focus Knobs

• Two flavors of these
• Course
• Fine
• Some microscopes control the course and fine
  focus with one knob.

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Stage Adjustment Knobs

• Controls the stage movement.
• Top knob controls forward and back movement.
• Bottom knob controls left and right movement.

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Cleaning the Microscope

• Keeping the microscope clean is extremely
  important.
• Use a clean, lint-free lens tissue.
• Dont use a dry tissue to clean the lenses. Use
  a moist tissue first and then a dry one to mop up.

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Cleaning...

• Start by cleaning the illuminator.
• Move to the condenser
• Next clean the objectives, but clean the 100x
  last because there may be oil on it. Once you
  have cleaned the 100x, get a new tissue.

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Cleaning...

• Clean the oculars
• Lastly, wipe off the stage. Clean it last
  because it usually has the most dust and oil.
• Make this a daily routine.

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