Project%201%20Frog%20Gels:%20Analysis%20of%20Skin

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Project 1Frog Gels Analysis of Skin

• John Donich
• Claudia Morales
• Kevin Berry

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Project description

• Goal to describe the set of proteins in 7.5
  gels and 12 gels
• Are males differentiable from females?
• Are individuals differentiable?

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An ideal model

• There is little variation between frogs of the
  same sex
• Theres a distinct diference in the set between
  sexes
• A protein that shows in only one of the sexes

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Method

• Measured relative mobility of protien bands
• Plotted standards relative mobility vs. Log
  Molecular Weight
• Chose a model and fit the data
• Looked for patterns within individuals and
  between sexes

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Choosing models

• Linear Model for 7.5 gels
• Only 3 Degrees of Freedom (5 Standards)
• Linear Model had smallest confidence interval
• Cubic Model for 12 gels
• 5 Degrees of Freedom (7 Standards)
• Cubic and Linear models had similar confidence
  intervals, but R2-adj better for cubic (99 vs
  96)

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Conclusions

• The variation among individuals is present
• The sex of a frog cannot be determined by protein
  gel analysis
• There is no single protein that distinguishes
  sexes

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