Hydrolysed Plant Protein

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PRO-AM
NEW

• Hydrolysed Plant Protein
• Pre-digested protein source
• for better absorption

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PRO-AM PRODUCTS

• Our 3 new products, under the PRO-AM umbrella
  brand contain pure pre-digested protein from
  de-oiled cakes of plant origin.
• PRO-AM is hydrolysed using an enzymatic
  hydrolysis process and contains mainly di- and
  tri-peptides.

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PRO-AM PRODUCTS

• PRO-AM Racing
• PRO-AM PROtein-prep
• PRO-AM Boost

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Main Indications for Use

• Improved lean muscle conditioning
• Delayed fatigue
• Quick recovery following strenuous exercise
• Lower weight loss following racing / training
• Branched chain amino acids
• Pre-digested protein for horses on restricted
  diets following surgery, injury or digestive
  disorders
• Increased protein during lactation

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Hydrolysis

• Hydrolysis is a process where the protein
  undergoes a chemical reaction in the presence of
  an enzyme by which the protein is converted into
  smaller chain proteins called peptides.
• Whole protein is insoluble in water but peptides
  are soluble in water.

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Peptides

• Peptides are small chain proteins. They follow
  after specific enzyme action cuts the long chain
  into mainly small chains of just two or three
  amino acids called di- and tri-peptides.
• These peptides are the precursors of amino acids.
  
• Small peptides such as di- and tri-peptides are
  directly absorbed into the blood stream.
• Poly and longer chain peptides undergo a further
  reaction in the small intestine and get absorbed
  as di- and tri-peptides.
• The peptides in the blood stream are either
  broken down into amino acids or used, as they
  are, to form various proteins to build the cells
  in the body.

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Amino Acids

• Essential as well as other amino acids are
• present in this product

The essential amino acids are marked with
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Protein Deficiency

• A deficiency of dietary protein may represent
  either an inadequate intake of high-quality
  protein or the lack of a specific essential amino
  acid.
• The effects of deficiency are generally
  non-specific, and many of the signs do not differ
  from the effects of partial or total energy
  restriction.
• In general, the horse will have poor quality hair
  and hoof growth, weight loss, and poor appetite.
  In addition, there may be decreased formation of
  Hgb, RBC, and plasma proteins.
• Milk production is decreased in lactating mares.

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Protein Distribution

• Protein is responsible for the formation, growth
  and maintenance of all body tissues.
• Protein represents about 80 of the horse
  excluding fat and water contents.
• Approximate distribution throughout the body
• Muscle 56
• Bone 12
• Hide/Hair 10
• Adipose Tissue 8
• Digestive Tract 4

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Crude/Digestible Protein

• Most feeds and supplements declare Crude Protein
  by law.
• Digestible Protein is the bit thats actually
  absorbed by the digestive tract.
• Old leather contains high levels of crude protein
  but it will not be very digestible.
• Generally, the older the pasture the less
  digestible protein it will contain.
• The younger and fresher the pasture the more
  digestible protein it will contain.
• Basically the higher the fibre the less
  digestible protein.
• Low fibre feedstuffs such as oilseed meals
  improve digestible protein values but fibre is
  absolutely essential too.

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Feed Digestion

• Digesta Saliva
• Stomach Gastric Juices Protein, Fatty Acids
• Small intestine Intestinal Juices Protein,
  Fatty Acids,
  Carbohydrates
• Caecum Micro-Organisms Digestive Juices
  Volatile Fatty Acids, Electrolytes
  other residues.
• Large Intestine Water, Residues of VFAs and
  Residue of Protein
• Rectum
• Faeces

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Protein Challenge

• Horses are able to make most non-essential amino
  acids from the other feed components, within
  their digestive tract.
• Stress, Exhaustion, Disease, Malabsorption,
  Surgery/Injury (as examples) have a negative
  effect on the bodys ability to cope with
  immediate requirements making other amino acids
  conditionally essential so that for least
  damage to occur (under special conditions) they
  are best replaced quickly through nutritional
  intervention.

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Conditionally Essential - example

• Glutamine forms approximately 50 of the amino
  acid pool in the body
• When peptide transported it
• (a) doesnt degrade in solution
• (b) does reach blood in concentrations needed to
  replenish stores
• (c) regulates glucose metabolism by converting to
  glucose without upset to hormones insulin or
  glucagon.
• (d) preserves lean muscle mass in carbohydrate
  depleted (ie near exhausted) athletes because of
  the point c above and fuelling muscles by
  converting into muscle glycogen reserves.

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Conditionally Essential - continued

• (e) during carbohydrate depletion glutamine will
  help burn body fat
• (f) it contributes to Nucleic Acid synthesis
  which is essential to all new and replacement
  cell growth
• (g) it is the source of Glutamic Acid which makes
  GABA (gamma amino butyric acid) which boosts
  mental awareness and relaxes the body. Glutamic
  Acid with Glycine and Cysteine form the
  tri-peptide GSH which is of significant value
  in imune and stress response.
• (h) it helps resist the passage of bacteria from
  the intestine to the bloodstream.
• (i) it supports production of Gluthatione which
  is a powerful antioxidant.

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Depletion

• When Glutamine levels are depleted the body will
  recycle it by breaking down tissue which will
  release it and other proteins but that means a
  loss of muscle condition.
• PRO-AM users note the lower levels of weight loss
  despite the rigours of training and racing.
• During rest the body makes this Glutamine from
  other amino acids but that happens at natures own
  pace which is not necessarily ideal for hard
  working race and competition horses.
• It is proven that strenuous exercise lowers the
  blood concentration of Glutamine.

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Over-Training

• Generally if amino acid reserves are not restored
  sufficiently fast enough so theres enough of a
  reserve to take the horse into the next training
  session over-training syndrome will occur.
• Push on and hell just loose weight, condition,
  possibly suffer injury, digestive problems and
  will likely loose the appetite for work.
• Balance the exercise programme with sufficient
  digestible protein and carbohydrates for energy.

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Glycogen Loading

• In human studies of athletes performing to
  Olympic standards it was confirmed that soluble
  proteins
• Are available faster for the body
• They can be used immediately by the body and
  taken in combination with carbohydrates close to
  the period of exercise they stimulate the
  production of insulin so that the muscle can load
  up on blood glucose faster and better.
• The faster the glucose is taken up by the muscle
  the faster the muscle produces glycogen so
  improving energy reserves.

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Endurance

• Normally its not good to take on a high protein
  load prior to or during exercise as whole
  proteins need to be digested.
• You wouldnt want a concrete brick in your
  stomach just before take off!
• Hydrolysed and Pre-Digested Protein overcomes
  that obstacle.

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CPK Creatine Phosphokinase

• CPK is an accurate measure of current muscle
  activity and so is a good indicator of what
  happened in the morning workout.
• Low levels represent no particular worrying signs
  but high levels sometimes into 4 - 6 digit
  figures indicate underlying muscle damage.
• CPK levels rise and fall through larger ranges
  earlier in preparation and tighten into a
  narrower range as the horse improves fitness
  levels.
• In those human trials mentioned earlier CPK
  levels were 5 times lower in athletes using
  hydrolysed pre-digested protein compared to
  controls taking only carbohydrate drinks. Very
  significant result.

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Summary

• Di and tri-peptides work as stable, fast, soluble
  sources of protein.
• They are builders and preservers.
• In building strength and endurance they are both
  anabolic (muscle building) and anti-catabolic
  (muscle protecting).
• They are not doping substances.