Toxicity Sensing Device for Inhaled Materials

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Toxicity Sensing Device for Inhaled Materials

• Biochemical Engineering Journal
• M. Riley, K. Jordan, M. Cox

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Introduction

• Currently there is no portable way to detect
  toxicity in the air.
• Device uses cells to measure metabolic
  activities.
• Device does not identify specific toxin.

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Procedure

• Monolayer of cells on the bottom of device.
• Lung cells are used.
• Rat is the source of cells.
• Cells came from culture.

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• Zink is used as the toxin.
• The device exposes cells for one hour.

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• Cell metabolism measured by the utilization of
  WST-1 dye.
• The healthier the cell, the more dye utilized in
  metabolism.
• The more dye utilized the greater the color
  change. (orange to red)

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• Color change detected by photometric sensor.
• Orange to red corresponds to blue light absorbed.
• Sensor detects blue light that is absorbed.
• Dye is sensitive to light. Exposure time and
  light intensity was kept to a minimum.
• Utilization of dye measured in sensor output
  voltage (mV).

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Results

• Exposure to toxins decreased amount of cell
  metabolism and metabolic rate.

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Critique

• Not very clear on effects of non standard
  conditions.
• Not very clear on how to make this device
  portable and efficient.

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The End.