Depicted on the following slides are water samples taken from scuba diving rinse tanks at a dive fac

1

• Depicted on the following slides are water
  samples taken from scuba diving rinse tanks at a
  dive facility on Bonaire, Netherlands Antilles
  October, 2007, for 3 days.
• Equipment rinse tank A was drained, cleaned
  with bleach and rinsed each morning, before
  filling with water and sampling (800 AM)
• Equipment rinse tank B was drained and rinsed
  each morning, before filling with water and
  sampling (800 AM)
• Water samples were taken from both equipment
  rinse tanks at 100 PM and 500 PM
• Water samples were taken from a regulator rinse
  tank daily at 800 AM, 100 PM and 500 PM,
  but we did not know when this tank was drained
  and refilled (by dive facility personnel and
  other divers)
• Water samples were taken daily from the hose
  used to fill all rinse tanks
• On two days water samples were taken from
  buckets on dive boats used to rinse/store masks
• 0.25 ml of each water sample was spread on
  Terrific broth-agar plates plates were incubated
  at 37oC overnight and photographed

2
On all days, water samples from the hose
(throughout the day) and from equipment rinse
tanks A and B at 800 AM, after draining,
cleaning with bleach (A) or not cleaning with
bleach (B), rinsing and refilling did not
produce any bacterial colonies. Representative
plates are shown below.
Hose Day 1, 1 PM
Hose Day 2, 8 AM
Hose Day 3, 8 AM
Tank B Day 1, 8 AM
Tank A Day 3, 8 AM
3
Day 1
Regulator tank 5 PM had no colonies (not shown)
Regulator tank 8 AM
Regulator tank 1 PM
Tank B 1 PM
Tank B 5 PM
Tank A 1 PM
Tank A 5 PM
4
Day 2
Regulator tank 8 AM
Regulator tank 5 PM
Regulator tank 1 PM
Tank A 5 PM
Tank B 5 PM
Tank A 1 PM
Tank B 1 PM
5
Day 3
Regulator tank 8 AM
Regulator tank 1 PM
Regulator tank 5 PM
Tank B 5 PM was empty and samples were not
taken
Tank A 1 PM
Tank B 1 PM
Tank A 5 PM, while filling
6
Mask rinse/storage tank 4 PM
Day 1
Day 2